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Tissue Sampling Articles & Analysis: Older
44 articles found
FFPE blocks not only preserve the tissue architecture but also allow for long-term storage and easy transportation, making them invaluable in various research contexts. ...
They are a diverse group of molecules, often existing in low concentrations within complex biological samples like blood, tissues, or cells. This is where advanced analytical techniques become essential. ...
While each has its strengths, the combination of these methods—ImmunoFISH—offers a unique and comprehensive approach to investigation at the cellular and tissue levels. Understanding the Techniques FISH is primarily utilized for detecting and localizing specific DNA sequences on chromosomes. ...
The Frozen Section Technique The process of preparing frozen sections involves several key steps that ensure the preservation of cellular architecture and molecular integrity. Tissue samples are first harvested from the lungs of C57 mice, often following euthanasia in accordance with ethical guidelines. The lungs are then rapidly cooled in an optimal cooling ...
A Customized Rabbit Cytokine Assay is a specialized service designed to measure the concentration of specific cytokines in rabbit samples. Unlike off-the-shelf kits, these assays are tailored to the unique requirements of the research project. This customization can include the selection of specific cytokines, the type of sample (serum, plasma, ...
Discover how matched tumor-normal sequencing can help clinical researchers detect the somatic origin of variants with certainty. In the era of precision oncology, it has become increasingly common for patients diagnosed with cancer to undergo tumor sequencing. Identifying the mutations that make up a tumor’s genomic landscape can help guide selection of targeted therapies and inform ...
Unveiling the Process: Key Stages Sample Collection: Precision in collecting samples from the primary infection site augments detection rates substantially. Sample Pre-Treatment: Different samples necessitate distinct pre-treatment strategies—liquefaction for sputum, dewaxing for FFPE samples, and ...
This technology identifies the diversity and specificity of T cells by measuring the sequences of the rearrangement regions of the TCRβ chain or α chain genes.(1) Sample Preparation: Total RNA or DNA is extracted from blood, tissue, or other samples enriched with immune cells.(2) Amplification: PCR amplification is performed using specific ...
After the bait protein reacts with the target protein, a band corresponding to the target protein can be detected depending on the bait protein used.Experimental Materials1. Samples: Cell or Tissue Extracts2. Western Blot Kit With Ubiquitination-Specific Antibody and Target Protein-Specific Antibody3. ...
Extraction of Cells or TissueCollect cell or tissue samples and lyse the samples with an appropriate cell lysis buffer (containing protease inhibitors) to release proteins.2. Sample Pre-TreatmentSamples can be broken down by sonication or centrifugation to break cell nuclei. ...
When it comes to detecting protein ubiquitination, Co-IP can be used to determine whether the target protein is ubiquitinated, and its interaction with ubiquitin protein.Analysis Workflow1. Sample PreparationCollect cellular or tissue samples, and lyse the cells with appropriate cell lysis buffer to extract proteins.2. ...
Therefore, the content of collagen can be indirectly estimated by determining the hydroxyproline content in the sample.(2) The method is to acid hydrolyze the sample, then react with Chloramine-T and p-Methylaminobenzaldehyde (PMB). ...
Biocompatibility testing evaluates the effect of a medical device on human tissues to determine whether it can cause biological reactions such as toxicity, physiological, immune, or mutation. ...
Metabolomics Research Methods Metabolomics collects samples through established metabolomics research methods, and subsequently uses techniques to obtain metabolites, determine whether there are statistical differences in metabolites between groups and perform further analysis. ...
The results show that most common pediatric brain tumor samples can be differentiated with DMS. DMS shows promise in becoming and additional instrument for real-time diagnosis of brain tumors. (The header image for this post shows an illustration of the laser sampling unit. The system is capable of vaporizing small tissue ...
These innovative approaches include genetic testing, next-generation sequencing, and biomarker analysis, which allows for more accurate and early detection of rare diseases by analyzing a patient's genetic makeup or identifying specific biomarkers in their blood or tissues. Genetic testing is one such breakthrough in diagnostics that has revolutionized the field of rare ...
They break down proteins, easing the isolation of DNA from cellular or tissue samples. Cell Culture: In cell culture processes, Native Porcine Pancreatin is instrumental for detaching adherent cells. ...
CD Genomics’ TCR-Seq service can be applied to various sample types, including blood, tissue, and cells, to obtain high-quality TCR sequences that enable researchers to gain a comprehensive understanding of T-cell clone diversity and clonality. ...
The IVS proprietary technology allows the growth of engineered tissues in 3D to mimic healthy and disease-naive human tissues, which enables the gap to be bridged between cell based assays and studies using isolated organs, tissues or animal models. ...
Enzyme synthesis and secretion in normal tissues are enhanced. In the case of tissue damage, the activity of serum is increased. The reason is the increase in the number and activity of enzymes in tissue cells. The induction of enzymes increases the synthesis of enzymes in tissue cells. ...