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Cell Culture Articles & Analysis: Older
106 articles found
In modern biopharmaceutical manufacturing, downstream processing development has become one of the most critical and resource-intensive stages. While upstream cell culture or fermentation focuses on producing the target biomolecule, downstream steps transform crude harvests into highly purified and stable therapeutic products. ...
Abstract Cannabis is used by an estimated 192 million people around the world. Most people use cannabis through the inhalation of cannabis smoke, which contains combustion by-products that can negatively affect lung health. Knowledge of these risks has led to a growing interest in cannabis vaporizers, which heat the dry cannabis flower without burning. Vaporizing cannabis still releases ...
Immortalized human podocytes represent a significant advancement in the field of renal research, offering scientists an unprecedented opportunity to study kidney function and pathology. These specialized cells play a crucial role in the filtration barrier of the kidneys, and their immortality allows for extended experimentation beyond the limitations of primary ...
The Significance of CHO Cells CHO cells are favored for their eukaryotic nature, which allows for proper post-translational modifications, essential for the functional integrity of antibodies. ...
The growth characteristics of CHO cells, combined with their ability to be cultured in various systems, make them suitable for large-scale production. ...
This assay plays a pivotal role in understanding how drugs are absorbed in the gastrointestinal tract and aids in predicting their bioavailability. Background of Caco-2 Cells Caco-2 cells are derived from human colorectal carcinoma and possess characteristics that mimic intestinal epithelial cells. When cultured, these ...
They can be produced in microbial systems such as yeast or bacteria, which reduce the complexity and cost associated with mammalian cell culture. Reduced Immunogenicity: Due to their smaller size and unique structure, single-domain antibodies tend to elicit a lower immune response in humans, making them safer for therapeutic applications. ...
Human dermal fibroblasts-neonate (HDF-n) are specialized cells derived from the dermis layer of neonatal human skin. These fibroblasts play a crucial role in skin structure, function, and repair. ...
Purity: Assessing the purity of the cell bank is essential to rule out contamination with other cell types, microbial organisms, or viruses. ...
In vitro testing involves studying immune responses in controlled laboratory environments using cell cultures. This method allows researchers to assess the direct effects of substances on immune cells and mechanisms. By analyzing changes in cell proliferation, cytokine production, and apoptosis, scientists can gather crucial data ...
NAD is a vital coenzyme found in all living cells. Its structure comprises two nucleotides joined by phosphate groups: one nucleotide contains an adenine base, while the other contains nicotinamide. ...
In vitro tests often involve using cell cultures or biological fluids to assess how drugs are absorbed and metabolized. ...
They play a critical role in RNA interference (RNAi), a biological process used by cells to regulate gene expression and defend against viral infections. ...
What are Colorectal Cancer Cell Lines? Colorectal cancer cell lines are cultured cells derived from tumor tissues of patients diagnosed with colorectal cancer. ...
Unlike primary cells that have a limited lifespan, stable cell lines offer a consistent and renewable source of cells, making them invaluable for long-term studies and industrial applications. ...
They are synthesized and secreted by a range of immune cells, including monocytes, macrophages, T cells, B cells, and natural killer (NK) cells, as well as certain non-immune cells like endothelial cells, epidermal cells, and fibroblasts. ...
They exhibit high levels of keratinocyte markers, which are essential for maintaining the structural integrity and function of the corneal epithelium. These cells can be cultured in vitro, allowing researchers to study their behavior under controlled conditions. ...
Since the production of recombinant protein vaccines involves the growth and expression of proteins in host cells (such as E. coli, yeast, mammalian cells or insect cells), the use of appropriate culture mediums is critical to successfully produce high-quality vaccines.Media component analysis is a crucial step in biotechnology ...
The most important and critical step in cell culture is the selection of an appropriate growth medium for in vitro culture. ...
However, the zebrafish (Danio rerio), also known as zebrafish, has emerged as an important new cancer model due to its small size, brood weight, and rapid maturation time, complements models that can traditionally be achieved in mice and cell culture systems. A wide range of assays can be performed in this model, from target discovery, target validation or ...