Dna Double Stranded From Plasmid Articles & Analysis: Older
9 articles found
Gene regulation is a complex and tightly controlled process that plays a crucial role in various biological functions, including cell growth, differentiation, and development. CRISPR technology has revolutionized the field of genetics and molecular biology by enabling precise editing of the genome. In recent years, CRISPR activation (CRISPRa) has emerged as a powerful tool for upregulating gene ...
Bioproduction is a critical element in the creation of many vaccines, therapeutics, and other biopharmaceuticals. A central facet of this process involves the development of stable cell lines that are capable of producing these vital products in abundance and with consistency. This article will explore the in-depth process and importance of stable cell line development in bioproduction. The ...
Ionic channels, which are proteins present in the membranes of all living cells, are gaining significant importance in the fields of bio-medicine and biotechnology. They are responsible for the transmission of signals across cell membranes and are instrumental in multiple biological processes. This raises the demand for a systematic mechanism that can control their expression. The customized ...
Induced pluripotent stem cells (iPSCs) can be generated from a wide range of fully differentiated cells and, under optimal conditions, can potentially be driven to differentiate into virtually any fate. Induced stem-like cells not only provide an alternative to embryonic stem cells but more importantly represent a powerful tool for drug development and disease modeling. Methods to induce ...
Introduction to target region sequencing Since the introduction of Sanger sequencing in 1977, genetic sequencing has been greatly improved with costs simultaneously falling. With the ability to rapidly produce large volumes of sequencing data, next-generation sequencing (NGS) enables researchers to obtain whole genome or targeted regions of samples. Targeted region sequencing is preferred by ...
The biological function of restriction enzymes is derived from the defense of foreign DNA from invading cells. The restriction-modification system of bacteria makes the DNA bases of bacteria to be methylated and modified, so that they are not recognized and cut by their own restriction enzymes. ...
RNA is a hydrophilic, negatively charged macromolecule, and its ability to autonomously transmembrane is limited. Therefore, how to efficiently deliver mRNA to the cytoplasm and play a corresponding role is one of the key issues that limit the application of RNA therapy. The wide range of applications of therapeutic mRNA requires efficient and safe delivery methods. An ideal delivery system ...
The growing pipeline of cell and gene therapy therapies has led to an increase in demand for gene delivery vehicles such as plasmid DNA (pDNA), messenger RNA (mRNA) and viral vectors. They can be used as stand-alone treatments or can be used as starting materials required for further processing of cell and gene therapeutics. When used as starting materials, they have been shown to have a ...
Given the height of the bacteriophage M13 (3-4 nm), a single bacteriophage may have been imaged at 1720 cm-1 (C=O) and 1667 cm-1 (amide I) via PiFM. As is the case in many instances, PiFM images reveal features that are not clear in ...