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Dna Library Articles & Analysis: Older

12 articles found

Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications   

Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications  

While many researchers now default to enzymes with longer recognition sequences for routine cloning, HindII enzyme activity characteristics provide distinct advantages in specialized applications including methylation analysis, forensic DNA fingerprinting, and metagenomic library construction. Its relatively frequent cutting pattern (GTY↓RAC, where Y=C/T and ...

ByCreative Enzymes


Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications   

Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications  

While many researchers now default to enzymes with longer recognition sequences for routine cloning, HindII enzyme activity characteristics provide distinct advantages in specialized applications including methylation analysis, forensic DNA fingerprinting, and metagenomic library construction. Its relatively frequent cutting pattern (GTY↓RAC, where Y=C/T and ...

ByCreative Enzymes


Fosmid Library Construction: A Comprehensive Overview

Fosmid Library Construction: A Comprehensive Overview

Construction Process The process of constructing a fosmid library begins with the isolation of genomic DNA from the organism of interest. The quality and integrity of this DNA are crucial, as damaged DNA can lead to inefficient cloning and low-quality libraries. ...

ByCreative Biogene


Advancements in Sequencing Technologies: Transforming Pathogen Detection and Clinical Diagnostic   

Advancements in Sequencing Technologies: Transforming Pathogen Detection and Clinical Diagnostic  

An extraordinary attribute of long-read sequencers is their ability to directly sequence raw DNA/RNA samples, obviating the need for PCR amplification. Additionally, they exhibit an unbiased approach toward CG nucleotides and offer the direct detection and retrieval of methylation information. ...

ByCD Genomics


Genomic Library Construction Service

Genomic Library Construction Service

Creative Biogene is a biotechnology company specializing in custom genomic library construction services. With years of experience in service of genomic library construction, Creative Biogene can construct genomic libraries from nanogram quantities of genomic DNA or milligram quantities of the tissue. ...

ByCreative Biogene


Genomic Library Construction Service

Genomic Library Construction Service

Creative Biogene is a biotechnology company specializing in custom genomic library construction services. With years of experience in service of genomic library construction, Creative Biogene can construct genomic libraries from nanogram quantities of genomic DNA or milligram quantities of the tissue. ...

ByCreative Biogene


New Technology to Promote Drug Development-AI Technology

New Technology to Promote Drug Development-AI Technology

At the same time, these companies are constantly looking for new technologies for new drug development, such as high-throughput screening technology, DNA encoded chemical library, computer-aided drug discovery, and artificial intelligence. ...

ByBOC Sciences


Exploring the Magic of Tn5 Transposase

Exploring the Magic of Tn5 Transposase

This allows researchers to insert or delete specific DNA sequences with great precision. Applications of Tn5 Transposase DNA Sequencing: Tn5 Transposase is a fundamental component in various DNA sequencing methods, such as Next-Generation Sequencing (NGS) library preparation. ...

ByCreative Enzymes


SuperCut Series: Redefining Enzyme Tools for Precision Research and Applications

SuperCut Series: Redefining Enzyme Tools for Precision Research and Applications

Genomics Research: SuperCut enzymes find applications in specific DNA sequence cleavage, such as gene library construction, site-directed mutagenesis, and DNA fragment analysis. ...

ByCreative Enzymes


The Methods of Whole Genome Sequencing

The Methods of Whole Genome Sequencing

Whole Genome Shotgun Sequencing (WGS) WGS generally involves six steps, isolation of genomic DNA, random fragmentation of genomic DNA, size selection using electrophoresis, library construction, paired-end sequencing (PE sequencing), and genome assembly. ...

ByCD Genomics


When Respiratory Pathogen Metagenome Sequencing Meets Hybrid Capture Enrichment

When Respiratory Pathogen Metagenome Sequencing Meets Hybrid Capture Enrichment

By designing and synthesizing specific probes targeting the target region (human genome or microbial specific region, etc.), hybridizing with the target genomic library DNA containing the target region in the sample, capturing and then performing high-throughput sequencing using a mainstream sequencing platform. The sample nucleic acid is extracted and then a ...

ByCD Genomics


Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD

Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD

Microbiome analysis using 16S rRNA sequencing DNA in stool samples was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany). ...

ByKoBioLabs

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