Refine by
Expression System For Protein Expression Articles & Analysis: Older
74 articles found
The principle is to clone one or several antigen genes of a pathogen into host cells, causing them to express the corresponding antigen protein. These proteins are then extracted for use in the vaccine. ...
Cell culture is one of the major technologies in the life sciences. It is a general term used to remove cells, tissues or organs from animals or plants and place them in an artificial environment conducive to their survival and/or proliferation. The basic environmental requirements for optimal cell growth are: controlled temperature, substrate for cell attachment, appropriate growth medium and ...
Human brain has approximately 171 billion cells, of which slightly more than half (approximately 86 billion) are nerve cells. These 86 billion nerve cells are a diverse cell population with hundreds of specialized types and functions, but all originate from three neural cell lineages--neurons, oligodendrocytes and astrocytes. All three cell lineages originate from a pool of neural stem and ...
These approaches allow the targeted delivery of genes that can inhibit tumor growth or enhance the immune system's capability to recognize and destroy cancer cells. One promising strategy is to deliver genes that express tumor suppressor proteins or anticancer agents into cancer cells to induce cell death or inhibit their proliferation. ...
Differentially expressed proteins refer to proteins that show significant changes in their expression levels in different biological samples or under different treatment conditions. Observing such differential expression is crucial for understanding cellular physiology and disease mechanisms. For example, some proteins may be upregulated or downregulated in health and disease states, or between ...
Plasma proteomics is an emerging field of science dedicated to the analysis and understanding of the protein composition in the plasma and its functions. Plasma, as one of the most abundant biological fluids in the human body, contains tens of thousands of proteins, reflecting the health status, disease process, and treatment response of the body. Therefore, plasma proteomics holds great ...
The immune system plays a crucial role in protecting the body from harmful pathogens and foreign invaders. Immune receptors, which are protein molecules expressed on the membrane of immune cells, play a key role in regulating immune responses. ...
Protein-Protein Interaction Sequencing (PPI-Seq) is an emerging technology. Based on high-throughput sequencing, it allows researchers to explore protein interactions at the whole genome level.Technical PrinciplesThe basic principle of Protein-Protein Interaction Sequencing is to use the yeast two-hybrid ...
Brain tumors can be broadly categorized into primary and secondary (or metastatic) tumors. Primary tumors originate in the brain1, while secondary tumors spread from other body parts. Treatment options traditionally include surgery, radiation therapy, and chemotherapy. However, these approaches can be invasive and have severe side effects. The battle against brain tumors is being ...
Collagen, as one of the most abundant proteins in the human body, plays a key role in maintaining the integrity and function of tissue structures. With the development of bioengineering technology, recombinant human collagen (rhCollagen) produced by recombinant DNA technology has been widely applied in various fields such as medicine, cosmetics, and tissue engineering. Therefore, qualitative ...
Gene knockout is a procedure that inactivates the function of a specific gene, making it unable to express normally, through genetic engineering techniques. This operation can help us understand the function and role of this gene in an organism. Gene knockout can be achieved using various methods, including the CRISPR-Cas9 system, TALENs, or ZFNs, etc.Importance ...
Protein-Protein Interaction databases primarily record experimentally validated protein interaction data. Here are some key Protein-Protein Interaction databases:BioGRID(Biological General Repository for Interaction Datasets)BioGRID is a database focused on biological molecular interactions, including protein-protein, protein-DNA, and other types of interaction data.DIP(Database of Interacting ...
After obtaining the protein sequencing results, various analyses can be performed. These analyses help to reveal the function, structure, interactions and roles of the protein in the organism. The commonly used types of analysis mainly include:1. Sequence AlignmentAlign with known protein sequences to identify homologous proteins, which helps to infer protein function and evolutionary ...
Recombinant collagen is a type of collagen protein expressed in microorganisms, animal cells, or plants through genetic engineering technology. ...
ComprehensivenessIt can detect more proteins and peptides in the sample, providing more comprehensive proteome coverage.2. ...
What Is RNA Splicing? RNA splicing is a post-transcriptional modification process that occurs in eukaryotic cells. When a gene is transcribed, the initial RNA product is called pre-mRNA, which contains both coding regions (exons) and non-coding regions (introns). RNA splicing removes the introns from the pre-mRNA molecule and joins together the exons to form a mature mRNA molecule that can be ...
Disease relevant mutations can be recapitulated or even correct by introducing a point mutation with a single base change, or by inserting/deleting large gene sequences. These endogenous knock-in cell lines enable a clear understanding of the contribution of the gene or mutation to a phenotype. There are unlimited uses for point mutation cell lines at pre-defined loci, including functional ...
Creative Biogene has established various plant-based protein expression systems to provide recombinant protein production service for global clients. Using our state-of-art platform, we offer a complete service that begins from a specific gene to the purified protein, as well as different options for ...
One of the first steps in drug development and toxicity testing is creating cell models on which to evaluate the effects of chemical compounds on cellular, molecular and biochemical processes of interests. Stem cells or stem cell-derived downstream cells are the best choice to establish cell models to evaluate new compounds because they are patient-matched. Creative Bioarray provides customers ...
Finally, the fusion gene is induced and the expressed protein is purified. Technology Features Fusion genes can be expressed in prokaryotic or eukaryotic cells. Prokaryotic expression systems are characterized by short time and low cost, and are the main tools in research. ...