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Gene Mutation And Expression Articles & Analysis: This-Year
14 articles found
Introduction The BALB/C mouse is a widely used inbred strain in biomedical research, particularly in the fields of immunology, cancer research, and genetics. Among the various tissues studied, the kidney holds significant importance due to its complex structure and multifaceted role in physiological functions. A formalin-fixed paraffin-embedded (FFPE) block of the BALB/C mouse kidney serves as an ...
The rat thymus, an essential organ in the immune system, plays a crucial role in the development and maturation of T-cells. Investigating the thymus of rats provides valuable insights into immunological processes and disease mechanisms. One of the most effective methods for preserving and studying the cellular architecture of the rat thymus is through the use of Formalin-Fixed Paraffin-Embedded ...
In the intricate world of cellular biology, proteins are the undisputed workhorses. They are assembled based on a genetic blueprint, but their story doesn't end there. After synthesis, proteins undergo a vast array of chemical modifications known as post-translational modifications (PTMs). These changes act as molecular switches, fine-tuning a protein's function, localization, and stability. ...
These features resemble those of ESCs, indicating the reprogrammed cells have regained pluripotency. Pluripotency Markers The expression of specific pluripotency markers is another critical aspect of iPSC characterization. Stem cell surface markers such as SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 are routinely analyzed via flow cytometry or immunocytochemistry. Additionally, the ...
Optogenetics has emerged as a transformative tool in the field of neuroscience, allowing researchers to manipulate neuronal activity with unprecedented precision. At the heart of this technology lies the use of adeno-associated viruses (AAVs), which serve as robust delivery vehicles for light-sensitive proteins into targeted neuronal populations. This article delves into the significance of ...
In recent years, the field of gene editing has experienced groundbreaking advancements, with CRISPR/Cas9 emerging as a powerful technology for precise genetic manipulation. Among various delivery systems, lentiviruses have garnered significant attention due to their ability to introduce genetic material into both dividing and non-dividing cells, making them an ideal vehicle for gene therapy ...
Fluorescence In Situ Hybridization (FISH) and Immunohistochemistry (IHC) are two powerful techniques widely used in biological and medical research. While each has its strengths, the combination of these methods—ImmunoFISH—offers a unique and comprehensive approach to investigation at the cellular and tissue levels. Understanding the Techniques FISH is primarily utilized for detecting ...
In the realm of biological research, the ability to accurately observe and analyze cellular components is paramount. Our experienced team specializes in providing advanced in situ hybridization (ISH) services, employing both bright field and fluorescent techniques to deliver precise insights into tissues and cells. The utilization of bright field microscopy allows for clear visualization of ...
Research in the field of pulmonary biology often requires detailed analysis of lung tissues. One of the optimal methods for studying lung structures and functions in mice is the use of C57 mouse models, specifically through the preparation of frozen sections. This technique is vital for a variety of applications, including histological studies, immunohistochemistry, and molecular analysis. Why ...
In the rapidly evolving field of cellular biology and genetic engineering, the development of efficient and reliable cell lines is vital for research and therapeutic applications. One such advancement is the establishment of the Monkey DLL3 stable cell line using HEK293T cells. This article provides an overview of the significance of this cell line, its applications, and the methodology behind ...
Simple Summary The present study investigated possible administration of pectin derived from apple pomace as a beneficial and cost-effective feed additive to be used in trout culture. To achieve the aim, a range of parameters were measured including immune parameters (both at physiological and molecular levels), growth performance and digestive enzyme activity. The results showed promising ...
Exosomes, small vesicles released by cells, play an increasingly prominent role in intercellular communication and disease modulation. These nano-sized particles contain a diverse array of molecular cargo, including proteins, lipids, RNA, and metabolites, which serve as biomarkers for a variety of physiological conditions. Exosome lipidomics and metabolomics are key fields that focus on profiling ...
Nuclear receptors are a class of proteins that play a vital role in cellular communication, gene expression, and overall homeostasis within the body. They act as transcription factors, responding to various stimuli, including hormones, nutrients, and even environmental agents. Due to their crucial roles in physiological and pathological processes, nuclear receptors have emerged as significant ...
Double-stranded RNA (dsRNA) has gained significant attention in the fields of molecular biology, genetics, and therapeutics due to its pivotal role in gene silencing and the modulation of cellular processes. The production of dsRNA can be achieved through various methods, each offering unique advantages depending on the desired application. This article delves into the primary techniques for ...