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Microfluidic Articles & Analysis: Older
29 articles found
POCT technical principles include dry chemistry, colloidal gold, immunofluorescence, microfluidic chips, chemiluminescence, biosensors, and biochips. Colloidal gold is the most commonly used nanoparticle for optical labeling due to its ease of use, biocompatibility, and high visibility. ...
The most common methods include simple mechanical stirring, ultrasonic treatment, high shear mixing (HSM), high pressure homogenization (HPH) and microfluidics. Depending on the preparation methods of PLGA nanoparticles, amphiphilic, hydrophobic or hydrophilic drug molecules can be loaded into the core area of the nanoparticles or adsorbed on the surface of the nanoparticles, ...
Another advancement in microneedle patch technology is the use of microfluidic channels for active ingredient delivery. These channels allow for precise control over the release of active ingredients, ensuring that they are delivered in the right concentration and at the right time. ...
Particle size can be determined using laser diffraction, dynamic light scattering, or microfluidic imaging. The surface charge of the aluminum adjuvant depends on the pH and as the electrostatic repulsion decreases, larger particle sizes are observed as the pH approaches the zero charge point. ...
Biofabrication: Bio-ink can be used to create complex biological structures for research and development, such as microfluidic devices for drug delivery. Challenges and Future Directions Despite its immense promise, bio-ink technology is still in its early stages of development. ...
ByMatexcel
Using the laminar flow of the microfluidic chip and an external magnetic force, the reporter molecule and the detergent generate three fluid streams through the action of the magnetic force. ...
Subsequently, these library fragments can be amplified in situ by hybridization and extension from complementary adapters which are covalently attached to the surface of a glass microfluidic cell or a small bead (depending on the sequencing platform). All NGS instruments utilize a microfluidic device to contain the amplified fragments of the shotgun library, ...
The test will be developed on Nicoya’s Alto digital surface plasmon resonance (SPR) platform which allows for real-time interaction analysis powered by digital microfluidics (DMF) technology. According to the company, the SubB2M-based SPR test has the potential to be developed as a multi-cancer detection test that first detects the Neu5Gc cancer signal and then applies a ...
The main techniques include gradient dilution, micro-manipulation, fluorescence-activated cell sorting, microfluidics, and laser capture microdissection, etc. The core strategies include isolating individual cells and constructing sequencing libraries independently; the other is labeling-based single cell identification (Barcode). ...
Fabrication and assembly of the microfluidic device The moulds for microfluidic devices were fabricated in the cleanroom using standard photolithography. ...
We also heard from Dr Cees van Rijn, Professor of Nanotechnology and Microfluidics at the University of Amsterdam, about his team’s work on Vibrating Mesh Nebulisers and their opportunities for pulmonary delivery. ...
Here, we present spatial-CUT&Tag for spatially resolved genome-wide profiling of histone modifications by combining in situ CUT&Tag chemistry, microfluidic deterministic barcoding, and next-generation sequencing. Spatially resolved chromatin states in mouse embryos revealed tissue-type-specific epigenetic regulations in concordance with ENCODE references and provide ...
For example, biomedical engineer Rong Fan at Yale University in New Haven, Connecticut, developed a platform known as DBiT-seq16, which employs a microfluidic system that can simultaneously generate barcodes for thousands of mRNA transcripts and hundreds of proteins labelled with oligonucleotide-tagged antibodies. ...
However, the disadvantage of physical methods such as electroporation, microinjection, hydrodynamic injection, and microfluidic cell delivery is that it may cause cell death, and the scope of application is relatively ...
A breakthrough in nanotechnology accelerates vaccine production While COVID-19 hotspots have continued to emerge driven by the Delta variants, the data still shows that vaccinations are effective at preventing hospitalizations and deaths. While over 4 billion vaccine doses have been administered worldwide, only 27% of the world’s population and only 1.1% of people in low-income countries ...
Cartridges feature two major parts—the microfluidic chip “printed” on a test card and the molded cartridge housing. ...
ByFluxergy
Highlights Simultaneous transcriptomic and proteomic analysis on fixed tissue slides 10- and 25-μm spot size for varying resolution and near-single-cell analysis DBiT-seq can be conducted with common laboratory equipment and reagents DBiT-seq produces high-quality RNA sequencing data with high spatial resolution (10 μm) Summary This protocol describes the use of the ...
This technology uses hydrodynamic conditions termed microfluidic vortex shedding (µVS) that can be coupled to a brief electric field to gently permeabilize cells, allowing for the quick and efficient delivery of constructs like gene-editing complexes and nucleic acids into cells. ...
This approach also makes our platforms simple to use; no pumps or complex systems are required, as with microfluidic devices. RepliGut® Planar Our workhorse platform is a planar model (RepliGut® Planar) based on human stem cells that are expanded and then differentiated to recreate the human small intestine or colonic epithelium for compound screening. ...
Gene-modified cell therapies (GMCTs) represent the most effective therapeutic platform for many patients with advanced diseases including relapsed and refractory leukemia, non-Hodgkin lymphoma, and other blood cancers [1]. Specifically, chimeric antigen receptor T cell (CAR-T) therapies targeting CD19 have demonstrated remarkable responses and possibly cures in patients with advanced acute ...