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Targeted Multiplexed Protein Articles & Analysis: Older
102 articles found
What Is PROTAC Technology? Proteolytic targeting chimera (PROTAC) is a new type of drug design technology that works by inducing the degradation of target proteins. Unlike conventional small molecules that typically inhibit protein function, PROTACs facilitate the ubiquitination and subsequent degradation of specific proteins. This bifunctional molecule consists of two distinct elements: a ...
As we step into 2024, the global health landscape is increasingly focused on combatting viral infections, which continue to pose significant threats to public health. The COVID-19 pandemic has illuminated the critical need for innovative strategies in antiviral research, and scientists worldwide are embarking on groundbreaking projects to develop more effective therapies, vaccines, and preventive ...
GMP growth factors and cytokines designed for therapeutic manufacturing are an essential component of defined media. To date, the cell therapy industry has accepted GMP terms for this reagent class, despite the fact that there is no direct oversight by regulators. These proteins are intended for use in further manufacturing processes and do not come into direct contact with patients. In fact, ...
Peptidomimetics are structural analogs of peptides or proteins that mimic their biological activities. They are designed to overcome limitations associated with peptides, such as poor stability and bioavailability by preserving or enhancing their functions. Peptidomimetic development involves the design and synthesis of molecules that mimic three-dimensional structures and functional groups of ...
Glycosylation modification is a chemical modification process on biomolecules, involving the combination of sugar molecules with other biomolecules (usually proteins). This modification process plays a crucial regulatory role within the organism, particularly in terms of protein function and stability.Glycosylation modifications also impact antibodies, manifested in the following ways:Antibody ...
Ubiquitination is a post-translational modification process that involves various biological processes, including signal transduction, cell cycle regulation, cell stress responses, and cell apoptosis. It plays a crucial role in maintaining protein homeostasis, regulating various biological reactions in cells, and ensuring the normal physiological functions of organisms. Immunoprecipitation is a ...
CD BioSciences, a biotech company specializing in centrosomal study, has recently launched new services for anti-centrosome antibody development. The services help identify and track centrosomes in experiments, demonstrating the strength of anti-centrosome antibodies as a great tool for centrosome research. The centrosome is a key cellular structure that plays a crucial role in the organization ...
Mitochondria are a unique type of organelle present within cells, serving as the primary source of energy within living organisms. They are responsible for several life-sustaining processes, including energy production, reducing free radicals, and maintaining cellular signaling. Proteins within the mitochondria play a key role in these processes.Why Sequence Mitochondrial Proteins?The sequencing ...
The T cell antigen receptor (TCR) is a characteristic marker of T cells, playing a crucial role in the immune system. Each T cell typically expresses a specific TCR, which has a unique structure and sequence that can recognize different antigens, including bacteria, viruses, tumor cells, and other pathogens. Once the TCR binds to the antigen, the T cell initiates an immune response, generating ...
The Unique Value and Challenges of Peptide Antigens in Antibody DiscoveryChoosing the right antigen is crucial in antibody discovery. The method of using peptides as antigens has been around for over 60 years. With technological advancements, we now have more novel avenues to choose from, such as recombinant proteins, engineered cells, and DNA encoding proteins, all of which can display a variety ...
Western blot can accurately detect the presence and expression of specific proteins in samples, and it can also be used to detect protein post-translational modifications, such as phosphorylation, ubiquitination, etc. The overall workflow of Far-Western Blot technology is similar to Western Blot, but the detection of target proteins in Western Blot requires antibodies, while antibodies are not ...
Protein Ubiquitination is a crucial cellular biological process. It involves the binding of Ubiquitin protein to the target protein, thereby regulating the degradation, positioning, activation, etc., of the target protein. To detect protein ubiquitination, methods such as Immunoprecipitation (IP) combined with Western blot, mass spectrometry analysis, etc., are commonly used. Here are the typical ...
From protein immunoblotting to immunofluorescence, and then to FRET, antibodies are often an important determinant of successful and repeatable experiments. But we've never had a set "plan" to find the most suitable antibody. In this issue, the editor shares a 10-step "plan" for antibody selection.Determine the Name of the Target ProteinFirst, you need to be clear about what protein you need to ...
Biopharmaceuticals and personalized medicine are hot fields in the development of the pharmaceutical industry today. Proteins, peptides, and antibodies are important molecules in the body and are important objects of research in biopharmaceuticals and personalized medicine. With the continuous advancement of protein, peptide, and antibody sequencing technology, they are playing an increasingly ...
In the fields of cell biology and genetic engineering, gene knockout is a commonly used research method that can help us understand the function and role of a specific gene, and the efficiency of this process is critical. Gene knockout is a procedure that inactivates the function of a specific gene, making it unable to express normally, through genetic engineering techniques. This operation can ...
Custom mRNA production has emerged as a cutting-edge technology in the field of molecular biology, offering researchers the ability to tailor-make specific messenger RNA molecules for various applications such as vaccine development, gene therapy, and regenerative medicine. This innovative process holds great promise in unlocking new possibilities in scientific research, paving the way for ...
Recently developed CRISPR/Cas9-based screening methods have great potential for revealing the mechanisms and treatment strategies of human diseases, but there are still some biological studies that are not suitable for CRISPR knockout screening. CRISPRa and CRISPRi screening opens up many new possibilities. CRISPR/Cas9 PlatformCB provides custom libraries for any set of genes of interest, as well ...
Deletion of target proteins in cells and observation of their phenotype is one of the most common methods for characterizing target protein function. However, if the loss of the target gene affects cell survival, then the acquisition of conditional knockout cells is important. Conditional knockout cell lines provide better control of gene expression and provide a more physiologically relevant ...
Antibodies can often be used in multiple assays, but they do not perform equally well in all assays. This is particularly true for immunohistochemistry (IHC). IHC has now become an indispensable assay and is consistently performed in anatomic pathology to give specific diagnosis and subclassification of neoplasms. IHC is a complex assay, where the end result is influenced by a variety of ...
Post-translational modification (PTM) refers to the biochemical process where specific amino acid residues of a protein are chemically modified after its translation. These chemical modifications greatly expand the functional diversity of proteins, enabling them to participate in more cellular physiological processes. The following are some common post-translational modifications of proteins:1. ...