Westernblot Articles & Analysis: Older
34 articles found
Chemokines and their receptors form one of the most intricate signaling networks in immunology. Among them, CCR1 (C-C chemokine receptor type 1) has attracted attention for its dual role as both a guardian of host defense and a potential driver of chronic inflammation and cancer progression. For researchers, CCR1 is more than a seven-transmembrane G protein–coupled receptor (GPCR)—it ...
Matrix Gla Protein (MGP) is a small, vitamin K-dependent extracellular matrix protein that plays a critical role in the regulation of vascular calcification and bone development. As a natural inhibitor of pathological mineralization, MGP has attracted significant attention in cardiovascular and skeletal biology research. The development of recombinant MGP protein has enabled researchers to study ...
Induced pluripotent stem cells (iPSCs) have revolutionized the field of regenerative medicine and cellular biology. They are derived from somatic cells that have been genetically reprogrammed back into an embryonic-like pluripotent state. This transformation enables them to differentiate into any cell type, offering significant potential for disease modeling, drug discovery, and therapeutic ...
Recombinant protein expression is a cornerstone of modern biopharmaceutical development, providing the means to produce proteins with therapeutic potential, enzymes, and antibodies. Among various expression systems, Chinese Hamster Ovary (CHO) cells have emerged as the preferred choice for many researchers and pharmaceutical companies. This article delves into the significance of recombinant ...
Single-domain antibodies, also known as nanobodies or VHH antibodies, are a unique class of antibody fragments derived from the immune systems of camelids, such as llamas and alpacas. These small yet robust molecules present significant advantages in various applications, from therapeutics to diagnostics. This article explores the process of single-domain antibody production, its benefits, and ...
Differentially expressed proteins refer to proteins that show significant changes in their expression levels in different biological samples or under different treatment conditions. Observing such differential expression is crucial for understanding cellular physiology and disease mechanisms. For example, some proteins may be upregulated or downregulated in health and disease states, or between ...
Protein phosphorylation is an important post-translational modification process that involves adding a phosphate group to an amino acid residue in a protein, usually a serine, threonine, or tyrosine residue. Protein phosphorylation plays a key role in regulating various biological processes within the cell, including signal transduction, cell cycle, and metabolism. Therefore, detecting protein ...
Host cell protein (HCP) residual detection refers to the testing of proteins that may remain in the final product and originate from the production cell line (i.e., the "host") during the biopharmaceutical production process. This testing is crucial because these host cell proteins (HCPs) may impact the safety, efficacy, and quality of drug products.In the fields of biotechnology and ...
HCP (Host Cell Proteins) residual detection is a key quality control step in the field of biopharmaceuticals, aiming at ensuring that the proteins of the host cells used for producing therapeutic proteins in any cell-based production system are maintained at acceptable low levels in the final drug product.ImportanceHCPs are impurities during the production process, which may adversely affect the ...
Glycosylation site detection refers to identifying the location where glycosylation occurs in biomolecules. Glycosylation is a biochemical modification process that involves linking sugar molecules to other biomolecules (such as proteins, nucleic acids, or lipids), thereby affecting their structure and function. Glycosylation plays a critical role in organisms, but it may also be associated with ...
Collagen is the most abundant protein in tissues and organs, and glycated collagen refers to collagen modified by glycosylation. Glycated collagen mainly exists in various tissues in the human body, especially those rich in collagen, such as skin, bones, joints, blood vessels, and other connective tissues.Under normal physiological conditions, glycation occurs as a natural biochemical process, ...
Acetylation detection of a specific protein is a method used to determine whether and where acetylation modifications have occurred on a protein. Acetylation is a common post-translational modification of proteins, typically occurring on lysine residues of proteins, and plays a crucial role in regulating protein function, cell signaling, gene expression, and disease onset. For instance, the ...
Ubiquitination is a post-translational modification process that involves various biological processes, including signal transduction, cell cycle regulation, cell stress responses, and cell apoptosis. It plays a crucial role in maintaining protein homeostasis, regulating various biological reactions in cells, and ensuring the normal physiological functions of organisms. Immunoprecipitation is a ...
The Unique Value and Challenges of Peptide Antigens in Antibody DiscoveryChoosing the right antigen is crucial in antibody discovery. The method of using peptides as antigens has been around for over 60 years. With technological advancements, we now have more novel avenues to choose from, such as recombinant proteins, engineered cells, and DNA encoding proteins, all of which can display a variety ...
Western blot can accurately detect the presence and expression of specific proteins in samples, and it can also be used to detect protein post-translational modifications, such as phosphorylation, ubiquitination, etc. The overall workflow of Far-Western Blot technology is similar to Western Blot, but the detection of target proteins in Western Blot requires antibodies, while antibodies are not ...
Protein Ubiquitination is a crucial cellular biological process. It involves the binding of Ubiquitin protein to the target protein, thereby regulating the degradation, positioning, activation, etc., of the target protein. To detect protein ubiquitination, methods such as Immunoprecipitation (IP) combined with Western blot, mass spectrometry analysis, etc., are commonly used. Here are the typical ...
From protein immunoblotting to immunofluorescence, and then to FRET, antibodies are often an important determinant of successful and repeatable experiments. But we've never had a set "plan" to find the most suitable antibody. In this issue, the editor shares a 10-step "plan" for antibody selection.Determine the Name of the Target ProteinFirst, you need to be clear about what protein you need to ...
In the fields of cell biology and genetic engineering, gene knockout is a commonly used research method that can help us understand the function and role of a specific gene, and the efficiency of this process is critical. Gene knockout is a procedure that inactivates the function of a specific gene, making it unable to express normally, through genetic engineering techniques. This operation can ...
Tau protein is a microtubule-associated protein, mainly present in nerve cells, and participates in the stabilization of microtubules. In certain neurodegenerative diseases, such as Alzheimer's disease (AD), Tau protein may undergo abnormal phosphorylation and aggregation, forming so-called Tau tangles or neurofibrillary tangles.Ubiquitination is a kind of post-translational modification of ...
Co-immunoprecipitation (Co-IP) is an experimental technique used to detect the interaction between a protein and other proteins. When it comes to detecting protein ubiquitination, Co-IP can be used to determine whether the target protein is ubiquitinated, and its interaction with ubiquitin protein.Analysis Workflow1. Sample PreparationCollect cellular or tissue samples, and lyse the cells with ...