CD Genomics articles

Degradome sequencing, also known as parallel analysis of RNA ends (PARE) sequencing, is a cutting-edge technique that leverages high-throughput sequencing and bioinformatics to investigate the degradation products of mRNA. This method is particularly valuable for identifying the target genes regulated by microRNAs (miRNAs), which are small, endogenous non-coding RNAs approximately 22 nucleotides in length. miRNAs play crucial

In the quest to unravel the complexities of infectious diseases, understanding the intricate interplay between pathogens and their hosts is paramount. This requires a comprehensive analysis of gene expression and the regulatory mechanisms at play during infection. Traditional RNA sequencing (RNA-seq) has long been employed to investigate the gene expression profiles of microbial pathogens. However, recent advancements in RNA-seq technologies have introduced innovative methods that delve deepe

Operational Taxonomic Units (OTUs) serve as fundamental units in the realm of numerical taxonomy, particularly in the study of microbial ecology. These units can represent various biological classifications, including individuals, species, genera, or even higher taxonomic levels. The term "operational taxonomic unit" was introduced to address the discrepancies between traditional taxonomic classifications and the units used in numerical methods, which often lack comparability. This distinctio

The advent of next-generation sequencing (NGS) has revolutionized the field of microbiome research, particularly through the analysis of 16S rRNA gene sequencing. This technique allows for a comprehensive understanding of microbial communities, providing insights into their composition and functional potential. This article outlines best practices for the bioinformatics analysis of 16S rRNA sequencing, emphasizing the critical stages of data preprocessing and quantification.

When a ribosome is affixed to the polypeptide (protein) it is making, the ribosome-nascent chain complex (RNC) is the compilation of molecules that make up the ribosome. One of several methods can be used to halt the synthesis of the nascent polypeptide. RNCs are made and purified in labs to study the dynamics, biochemistry, folding, and interactions that the ribosome and proteins undergoing synthesis.

The ribosome, as a critical part of the central dogma, is a no