Epigentek Group Inc. services

Whole Genome Bisulfite Sequencing (WGBS) is a bisulfite sequencing method to detect in-depth DNA methylation across the entire genome, including methylation at CpG sites and less common non-CpG site such as CNG. WGBS is the gold standard for bisulfite based DNA methylation studies as the entire genome is sequenced with base-level detail into where every methylated cytosine is located.

Reduced Representation Bisulfite Sequencing (RRBS) is an enhanced bisulfite-based sequencing method utilized to detect up 7 – 8 million unique CpG sites, covering nearly all CpG islands, gene promoters, and most genetic regulatory elements, gene bodies, and repeated DNA sequences. Limited Time Special Offer: Receive our Enhanced RRBS service for just $539 / sample (with minimum 12 samples per lane)

Targeted Bisulfite Sequencing is an NGS method used to evaluate site-specific DNA methylation changes. The process is similar to pyrosequencing but offers a much higher throughput overall. Our next-generation sequencing platforms can deliver a great amount of useful DNA methylation information with publication-ready data parsed by our expert bioinformatics scientists. The methylation analysis at single-base resolution of individual cytosine in DNA is facilitated by bisulfite treatment of DNA followed by PCR amplification of targeted region, library construction, and sequencing of the amplicon regions. Specific primers are designed for the region of interest and cytosine methylation changes are evaluated within that region. Each DNA methylation site of interest receives a high-sequencing depth of coverage for accurate, quantitative and single-base resolution data output.

Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying protein-DNA interactions and can be used to determine whether a specific protein binds to a particular sequence of a gene, such as the target sequence of a transcription factor or to compare the levels of histone methylation associated with a specific gene promoter region between normal and diseased tissues. Identifying the genetic targets of DNA binding proteins and revealing the mechanism of protein-DNA interaction is crucial for understanding cellular processes.