Gearbox Biosciences products

Gearbox Biosciences, a spin-off from the University of Tartu, Estonia, is pioneering antibiotic-free recombinant protein production through its innovative Pop-Out-Plasmid Technology. This advanced system eliminates the need for traditional selection markers like antibiotics and chemical inducers such as IPTG in E. coli cultures. By integrating the gene of interest directly into the host chromosome, our technology allows for stable maintenance during biomass growth. The subsequent production phase is initiated by a simple temperature increase, inducing a batch of molecular events that result in robust protein expression with increased scalability and efficiency. This decoupling of growth and production stages enhances yield while minimizing environmental risks associated with antibiotic use. Our work is supported by strategic collaborations and grants, allowing us to extend our technology for plasmid DNA production, critical in the realm of gene and cell therapy.