Gene Codes Corporation services

The forensic version of Sequencher gives you special functions for DNA-based identification. Using Sequencher’s Templates, which can help to support your standard operating procedures, you ensure your analysts are all using the same parameters and settings in their projects. We have even provided you with the Cambridge Reference Sequence (rCRS) as an example template in every installation. Consensus calling is important so you have a choice of including Consensus by Confidence and Consensus to Forensic Standards using the rules established by AFDIL. Reference Sequences are more than just a collection of A, C, G, and T, they set the base numbering and directionality of the contig. That means that, if you are working with the rCRS, then the location of your sequence variations is known absolutely. We also created a special numbering system so that insertions relative to the Reference Sequence don`t perturb the sequence numbering.

When you are working with NGS data, whether it is DNA-Seq or RNA-Seq, you will want the best algorithms. For speed, BWA-MEM is able to give you reference-guided alignments with genome sizes up to human genome size and beyond. Despite its speed, it still has a small memory requirement. For a feature-rich program able to deal with regular sequences, spliced sequences, methylation-tolerant alignments, SNP-tolerant alignments,  and RNA-I tolerant alignments, then GSNAP is the algorithm of choice. You can build the indexes for these programs, re-use them, or share them with colleagues, saving time and effort when working with large projects. And all without going near the command line.