IHC World products

The IHC-Tek Epitope Retrieval Solution is a ready-to-use formulation designed for the retrieval of antigens and epitopes across various protein types, including membrane, cytoplasmic, and nuclear proteins, from formalin-fixed paraffin-embedded tissue sections during immunohistochemistry procedures. This solution offers an advantage over standard citrate-based options due to its superior performance and reduced risk of tissue destruction compared to other high pH solutions. Ideal for universal application, it supports efficient antigen retrieval essential for accurate immunohistochemical analysis and is suitable for various research or diagnostic applications in the biomedical field where high-performance and reliable results are necessary.

The IHC-Tek Rabbit LSAB Detection Kit is developed for use with user-supplied Rabbit primary antibodies, facilitating the detection of antigens in tissue sections or cell preparations using light microscopy. This kit is particularly suitable for frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes, fixed culture cells, and also for whole mount IHC staining. A key feature of this detection system is its employment of a horseradish peroxidase (HRP)-labeled streptavidin and biotinylated secondary antibody system, which is widely recognized in immunochemical staining methodology. The enhanced binding strength of the kit's components offers improved sensitivity and reduced non-specific background staining, leading to a higher signal-to-noise ratio in comparison to the traditional avidin-biotin complex (ABC) method. Notably, when using the kit's Antibody Diluent as the primary antibody dilution buffer, the additional step of serum protein blocking may be omitted.