Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH products

Coxsackievirus B3 (CVB3) of the enteroviruses of the family Picornaviridae is associated with a variety of acute and chronic forms of diseases including meningoencephalitis, insulin-dependent type I diabetes (IDDM) (Curr Diab Rep. 2010;10(5):350-6) and, importantly myocarditis (Eur Heart J. 2013;34(33):2636-48). Although most enterovirus infections are subclinical, these viruses are capable of inducing severe acute myocarditis which may lead to life-threatening arrhythmias. With the introduction of the in situ hybridization technique and polymerase chain reaction CVB3 RNA was not only detected in endomyocardial biopsies of patients with acute and chronic myocarditis but also in those suffering from dilated cardiomyopathy (Proc Natl Acad Sci U S A. 1987;84(17):6272-6; New Engl J Med 2000; 343, 1388-1398).

Adiponectin is a protein of 30kDa which exists in several multimeric forms. About 0,01% of the proteins in human serum are adiponectin. The ADIPOQ-Gen is the gene mostly expressed by adipocytes. In spite of that the serum adiponectin concentration is negatively correlated with body fat mass. The protein consists of 244 amino acids and has a globulary domain which shows similarity to TNF alpha. The basic adiponectin form is a trimer beside the trime the existence of a hexameric and other higher molecular weight forms was proven. Adiponectin seems to influence the energy metabolism. In addition to the test system for the measurement of total human adiponectin Mediagnost offers specific test systems for the detection of adiponectin in mice and rats (see Animal Assays).

Growth Hormone Binding Protein (GHBP) consists of 238 amino acids and includes four sides for glycosylation and three disulfid bounds. In humans GHBP is formed by receptor shedding of the growth hormone receptor by a metalloprotease (ADAM17). Regulation of biological availability of GH is possibly the physiologic role of of GHBP. Thus, the half-life of GH is prolonged up to 100 minutes by binding to GHBP. GHBP correlates positively with the intraabdominal fat mass and is increased in type II diabetics with hyperinsulinaemia but not in type I diabetics. This correlation might indicate that GHBP is a suitable biomarker for the amount of visceral adipose tissue. The growth hormone binding protein ELISA allows the quantiative measurement of GHBP in serum and plasma. Recombinant human GHBP, produced in eukaryotic cell lines, is used for assay standardization.