3 services found

Orbit Discovery services

Engineering Services

Engineering soluble and membrane bound proteins. Our flexible peptide display platforms allow for screening directly against both cells and recombinant target protein. The Orbit team incudes experts on structural biology and protein production to ensure the quality of target protein for affinity screening. Orbit has specific capabilities in the production and screening of traditionally challenging integral membrane proteins, including GPCRs and ion channels.

Functional Screen Services

Using plate-based techniques, libraries of up to 50,000 peptides?derived from an affinity screen can be?screened against target?presented in functional cell models.? Generic activation of target?function is demonstrated directly in the screen and hits selected and?sorted. If rarer events are being assessed, or an abundance of leads?are required, microfluidic and droplet-based techniques can be?applied to screen libraries of up to 1 million peptides. These platform are ideal for searching for agonistic and antagonistic functional peptides, where the effect of the peptides on real life read-outs can be built into a screening cascade.

Affinity Screen Services

The Orbit Discovery Affinity Screening platform offers a proprietary technology enabling the construction of massive bead-based libraries with peptides displayed on their surface. Each bead carries a unique DNA sequence, which is translated into peptide using in vitro transcription translation (IVTT) performed in an emulsion (emIVTT). Each droplet of the emulsion acts as a micro-compartment which allows translation of the DNA on each individual bead to occur separately. The result is a library of unique beads each displaying thousands of copies of peptide, providing massive chemical diversity and the ability to perform rapid screens for high affinity binders against soluble or more complex target proteins, such as GPCRs and ion channels.