ProImmune Ltd. products

Purified immunodominant epitope peptide pools for precise monitoring of functional T cell responses. ProMix™ peptide pools from thinkpeptides consist of a select number of peptides representing the key immunodominant epitopes from a specific protein or pathogen, providing a comprehensive picture of the T cell response. Peptide selection is based on well-established and published epitopes with a wide range of HLA restrictions covering the most relevant HLA types in the population. Unlike peptide pools of protein spanning overlapping peptide libraries, ProMix™ pools allow for a much greater control and purity of its constituent peptide components and avoid overloading cell cultures with large numbers of irrelevant non-responding peptides that can contribute excessive contaminants from peptide synthesis processes.

Pro5® MHC Class I Pentamers the most consistent, most published commercial technology for detecting antigen-specific CD8+ T cells.  Pro5® MHC Pentamer. The beautiful rational design of Pro5® MHC Pentamers presents five MHC peptide complexes in a plane at one end of their pentameric coiled-coil core and five detection tags in a second plane at the opposite end of the core.

ProM1™ MHC Class I Monomers, biotin labeled enabling specialist assays for defined MHC Class I peptide target interactions.

ProT2® MHC Class II Tetramers track antigen-specific CD4+ T cells with the most comprehensive class II tetramer catalog range now also available for H-2 IAb, IAd, IAg7.

ProM2® MHC Class II Monomers, biotinylated enabling specialist assays for defined MHC Class II peptide target  interactions. Best in class MHC Class II Monomers: ProM2® Class II MHC Monomers are pre-biotinylated and can be readily adapted either to detect and separate single-antigen specific T cells or implement specialist solid or solution phase immunoassays for measuring interactions of predefined MHC Class II-peptide complexes with ligands such as monoclonal antibodies, recombinant T cell receptors, or other binding scaffolds, e.g. for use in screening of novel antigen-specific CAR T cell antigen receptors.

CD1d Tetramers the world’s leading product for tracking NKT cells. Detect NKT Cells with CD1d Tetramers: ProImmune is the leading commercial source worldwide for fluorescently labeled human and mouse CD1d tetramers. CD1d-lipid complexes bind to T cell receptors of NKT cells of a particular specificity (as determined by the lipid ligand used), allowing identification and enumeration of antigen-specific CD1d-restricted NKT cells by flow cytometry. Our tetramers show excellent brightness and experimental reproducibility. To avoid the difficulties associated with dissolving and loading the most commonly used ligand, alpha-Galactosyl Ceramide* (α-GalCer), our tetramers come pre-loaded. They can also be supplied empty for loading with your ligand of choice.

Human MR1 Tetramers detect MAIT cells with human MR1 tetramers. ProImmune is the leading commercial source worldwide for fluorescently labeled human MR1 tetramers. MR1 complexes bind to T cell receptors on mucosal-associated invariant T (MAIT) cells. MAIT cells have a particular specificity (as determined by the relevant MR1-binding small molecule ligand used), allowing identification, enumeration and isolation of antigen-specific MAIT cells by flow cytometry. Our tetramers show excellent brightness and experimental reproducibility. To avoid the difficulties associated with dissolving and loading the most commonly used ligands, 5-OP-RU and 6-FP, our tetramers come pre-loaded (not available in Australia and the United States). They can also be supplied empty for loading with your ligand of choice.

ProMix™ Peptide Pools purified immunodominant epitope peptide pools for precise monitoring of functional T cell responses.

A clear solution to a long-standing problem. Detecting and targeting specific MHC-peptide complexes is of material interest for understanding many disease conditions better and for developing novel therapeutic interventions. It has been very challenging to develop antibodies that bind selectively to an MHC-peptide combination over a more general MHC allotype.

Anti-Idiotype Ankyrons a game-changer for developing assays to track target-specific antibodies. Take the pain out of targeting the idiotype of your antibodies. Detecting and targeting specific antibody idiotypes is key for developing effective tracking assays for drug antibodies. Developing monoclonal and eventually recombinant anti-drug idiotype antibodies for such purposes has been possible but time-consuming and expensive.