Promega Corporation products

Cell Biology - Bioassays - Immune Checkpoint Bioassays

Promega FcyRIIb - Immune Checkpoint Bioassays Cells

The antibodies used to stimulate T cell activation via interaction with co-stimulatory receptors on the effector cells can be cross-linked via binding to FcγRIIb receptors on CHO-K1 cells. Based on the properties of the antibody tested, the Co-Stimulatory Immune Checkpoint (i.e., OX40, CD40, GITR and 4-1BB) bioassays can be conducted in a single genetically engineered cell system or dual genetically engineered cell systems. FcγRIIb CHO-K1 Cells are engineered to express human FcγRIIb, provided in cryopreserved thaw-and-use format. These are not reporter cells, and function solely to crosslink anti-CD40, anti-OX40, anti-4-1BB or anti-GITR antibodies.

Promega - Immune Checkpoint Bioassays Cells

4-1BB (CD137/TNFRSF9), a member of the tumor necrosis factor receptor superfamily, is an inducible co-stimulatory receptor expressed on T cells, natural killer (NK) cells and innate immune cell populations. When present on the cell surface, 4-1BB interacts with 4-1BB ligand (4-1BBL) and induces subsequent cell proliferation and production of interferon gamma (IFNγ) and interleukin-2 (IL-2), particularly in T and NK cells.