provitro AG products

The morphological structure of tissue can be depicted with high accuracy by means of histological sections. Staining results depend on numerous factors, including pH of the buffer solution, staining time and method of fixation. Haematoxylin, for example, is used to stain cell and tissue structures, such as cell nuclei, mitochondria, myelin, elastin and collagen fibres. Additional information may be obtained from counterstaining (differential staining), using a dyestuff in high contrast to hematoxylin stain. Counterstaining, using eosin, is a classical approach for which cationic structures are stained (e.g. proteins).

Normal TMA include normal tissue samples from a wide range of different human organs or matched organs from different species on one single slide.

Neoplastic (tumour) TMA include various neoplasms which are precisely graded, typed and classified according to WHO and/or UICC criteria.

Cardiovascular TMA include samples from cardiovascular normal tissue and myocardial infarction, myocardial hypertrophy of left heart/right heart and vascular tissue samples including classification of artheriosclerosis according to Stary.

Customised TMA include service of tissue microarray manufacturing and slide cutting according to customer’s needs. Additional immunohistochemistry service is available on request.

iConTMA are tissue slides for internal control of immunohistochemistry or in situ hybridisation. The slides contain specific positive and negative tissue spots providing enough space to add your tissue under investigation. The specificity of the reaction can be determined and the reactivity can be scored by comparing your sample with the iConTMA^® spots.

Endothelium is the cover term for the blood-oriented cells in the innermost layer of the vascular wall (intima). Functioning as physiological barrier to tissue, the CD 31- and vWF-positive cells express nitrogen oxide (NO) which plays a role in regulating the tonus of the vascular musculature. Damage to the endothelial cell layer has been assumed to be possibly a causative factor in the development of arteriosclerosis. Endothelium plays an equally important role in inflammatory processes and may be locally activated by various endogenous or microbial substances.