Reagen Biology LLC
5 products found

Reagen Biology LLC products

REAGEN - Glucose Assay Kit

Contact: reagenllc@gmail.com / anna@reagan.us REAGEN™ Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Glucose directly from food samples. The unique features of the kit are: High sensitivity and low detection limit (0.8 ppm) High reproducibility A rapid (30 minute), robust enzyme-based assay Procedure Overview REAGEN™ Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. The absorbance of each sample at 340 nm is measured using a spectrophotometer.

REAGEN - Fructosamine Enzymatic Assay Kit

Contact: reagenllc@gmail.com / anna@reagan.us REAGEN™ Fructosamine Enzymatic Assay Kit is a plate-based colorimetric assay for the determination of Fructosamine in serum samples. The kit uses a spectrophotometric assay to detect Fructosamine directly from serum samples, enabling researchers to detect Fructosamine levels in animal serum and other tissue matrices. The unique features of the kit are: High sensitivity and large assay range (1 - 8 mmol/L). A rapid (15 minute) and robust enzyme-based assay which does not require expensive instrumentation. High reproducibility. Introduction Fructosamines are stable carbohydrate-protein complexes that form in the blood by glycation reactions between sugars and primary amines. In diabetes, blood sugars are measured by either monitoring current blood glucose level or by glycated hemoglobin (HbA1c).

REAGEN - EHDV Detection Kit

Contact: reagenllc@gmail.com / anna@reagan.us Product Description The REAGEN™ EHDV Detection Kit provides a simple, rapid, sensitive and cost-effective enzyme-based immunoassay (ELISA) screening method for detectng EHDV from animal blood sample(serum or plasma) . Bluetongue virus (BTV), a member of the genus Orbivirus, produces similar symptoms, and differential diagnosis is important to prevent widespread outbreak and economic loss. The ELISA methodis specific, without cross-reactivity between the serogroups of BTV and EHDV.

REAGEN - Fructose/Glucose Assay Kit

Contact: reagenllc@gmail.com / anna@reagan.us REAGEN™ Fructose/Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Fructose/Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Fructose/Glucose directly from food samples. The unique features of the kit are: High sensitivity and low detection limit (0.8 ppm) High reproducibility A rapid (30 minute), robust enzyme-based assay Procedure Overview REAGEN™ Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample.

REAGEN - Casein Allergen Quantitative Test

Contact: reagenllc@gmail.com / anna@reagan.us INTENDED USE Veratox for Casein Allergen is intended for the quantitative analysis of food products, such as juices,sauces and sorbets,or environmental surfaces for the presence of casein. ASSAY PRINCIPLES The Veratox for Casein Allergen test is a sandwich enzyme-linked immunosorbenttion (Phosphate Buffered Saline, or PBS) by shaking in a heated water bath. Extracted casein residue is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound residue is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound casein residue.After a second wash, the substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities.