SBI System Biosciences, LLC products

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses RFP- EF1α-T7-hspCas9-T2A-RFP-H1-gRNA. Conveniently deliver Cas9 and gRNA with a single vector. Monitor transfection efficiencies with RFP, which is coordinately expressed with the hspCas9 gene via the T2A element. Drive Cas9 expression with the EF1α promoter, which provides medium expression levels in most cell types, including primary cells and stem cells. Express gRNA from the H1 promoter for maximum specificity and choice of targets. Ensure efficient import of Cas9 to the nucleus with N-term and C-term nuclear localization signals (NLSs).

Use this first-generation SBI EF1α-hspCas9 SmartNickase when you want to reduce off-target events, have transfectable cells, and prefer an intact vector. Conveniently deliver Cas9 Nickase and gRNA with a single vector. Reduce off-target activity. Drive Cas9 Nickase expression with the EF1α promoter, which provides medium expression levels in most cell types, including primary cells and stem cells. Express gRNA from the H1 promoter for maximum specificity and choice of targets. Ensure efficient import of Cas9 Nickase to the nucleus with N-term and C-term nuclear localization signals (NLSs).