Spherotech, Inc. products

The SPHERO™ Coated Particles are prepared either by passive adsorption or covalent coupling depending upon the intended applications. The avidin and biotin coated particles are prepared by covalent coupling. The antibody coated polystyrene particles are prepared using passive adsorption, yet they are stable for several years. The coated polystyrene particles from Spherotech can be used in a variety of applications. The Protein A coated polystyrene particles have been used for binding to IgG from human, mouse and rabbit serum. Glutathione coated polystyrene particles have been used for detecting the GST fusion proteins by flow cytometry.

We manufacture a wide variety of uniform amino functionalized particles ranging in size from 0.05 um to 20 um for covalent coupling of proteins or other ligands. Additional information can be found at FUNCTIONALIZED POLYSTYRENE PARTICLES product details.

The SPHERO Calibration Particles are designed for routine calibration of flow cytometers. By using these particles, the sensitivity and linearity of the system can be determined. The sensitivity of the instrument will help detect any dye or light contamination. The linearity of the instrument will determine if the logarithmic amps and PMTs are functioning properly.

Spherotech offers a wide variety of particles for determining if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO™ Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Particles for Absolute Cell Counting using a Single-Platform Flow Cytometric Method. Reference particles with known number of particles per mL for counting the absolute cell number by flow cytometry