Croyez - Model GMP IL -2 (Interleukin-2), Human Protein
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15.5-16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body`s natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
Sequence
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVL
ELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT with polyhistidine tag at the C-terminus
Source
Escherichia coli
Animal-free reagent and laboratory
Manufactured and tested under GMP guideline
Endotoxin level
Activity
Measure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is The specific activity of recombinant human IL-2 is approximately >2.5 x 107 IU/mg.
Measure by its ability to induce proliferation in NK cells. The ED50 for this effect is
Purity
>98% as determined by SDS-PAGE analysis. Purified by Ni-NTA chromatography.
Formulation
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Storage
Lyophilized protein should be stored at -20°C. This product is stable for one year upon receipt, when handled and stored as instructed. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Note
Please use within one month after protein reconstitution.
Croyez GMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP-certified facility.
The processes include:
- Testing and traceability of raw material
- Records of the maintenance and equipment calibration
- Personnel training records
- Batch-to-batch consistency
- Documentation of QA control and process changes
- Manufactured and tested under an ISO 13485:2016 certified quality management system
- Stability monitor of product shelf-life