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L-Homocysteine thiolactone hydrochloride

MCE China：L-Homocysteine thiolactone hydrochloride

Brand：MedChemExpress (MCE)

Cat. No.HY-101404A

CAS：31828-68-9

Purity：99.99%

Storage：4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Shipping：Room temperature in continental US; may vary elsewhere.

Description：L-Homocysteine thiolactone hydrochloride is an intramolecular thioester of Homocysteine. Homocysteine thiolactone hydrochloride prevents translational incorporation of homocysteine into proteins. L-Homocysteine thiolactone hydrochloride can induce elevated HHcy (hyperhomocysteinemia) in mice.

In Vitro：L-Homocysteine thiolactone hydrochloride (20 μM; for 24 h) shows significant increase in pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6[2]. L-Homocysteine thiolactone hydrochloride (100 μM; for 24 h) shows significantly higher ratios of nuclear/cytoplasmic NF-κB, indicating that Homocysteine (Hcy)-induced NF-κB activation and nuclear translocation[2].

In Vivo：L-Homocysteine thiolactone hydrochloride can be used to create models of hyperhomocysteinemia[2]. .f12{ font-size: 12px; } .fwb{ font-weight: bold; } .lh22{ line-height: 22px;; } .lh23 { line-height: 23px; } .pl13{ padding-left: 13px;; } .part { margin-top: 18px; } .mold-first-tit { width: 100%; height: 44px; line-height: 44px; background: #F9F7FB; border-bottom: 1px solid #EBE4F6; padding-left: 16px; box-sizing: border-box; margin-bottom: 17px; } .mold-second-tit:before { content:""; width: 6px; height: 6px; display: inline-block; border-radius: 50%; background: rgba(255,102,0,0.4); margin-right: 12px; position: relative; top: -3px; } .lft-border { border-left: 1px dotted #EBE4F6; padding-right: 12px; margin-left: 3px; box-sizing: border-box; padding-bottom: 12px; } /* .part .dec:last-child { border-bottom: 0; } */ .dec { margin: 10px 15px 0; padding-bottom: 10px; border-bottom: 1px dashed #EBE4F6; } .btm-border { border-left: 1px dashed #EBE4F6; } .text-bg { margin-top: 10px; background: #FFFBF1; padding: 14px; border-bottom: 0; position: relative; } .text-note-bg { margin-top: 10px; background: #FFFDF7; padding: 12px; border-bottom: 0; position: relative; } .text-note { width: 51px; height: 20px; line-height: 20px; background: #FFE2AA; text-align: center; border-radius: 0 0 8px 0; position: absolute; top: 0; left: 0; } .text-note-dec { margin-top: 15px;; } Induction of Hyperhomocysteinemia (HHcy)[2][3] Background Homocysteine (Hcy) is an amino acid that requires vitamins B12 and folic acid for its metabolism. Vitamins B12 and folic acid deficiencies lead to hyperhomocysteinemia (HHcy, elevated Hcy), which is linked to the development of diabetic retinopathy (DR), age-related macular degeneration (AMD), and Alzheimer’s disease (AD). Specific Mmodeling Methods Male C57Mice: cbs+/-; 3-5 weeks • C57BL6/J; 6-8 weeks of ageAdministration: 1 μL • intravitreally • 200 μM BL/6 mice (5 weeks old) Note (1) To avoid uncontrolled intraocular pressure increase, the volume of intravitreal injections is limited to 1 μL.(2) L-Homocysteine thiolactone hydrochloride is dissolved in water and a working solution of 10x is prepared by diluting 1 μL of stock solution (200 mM) to 100 μL with PBS. Then by injecting 1 μL of this working solution, 200 μM vitreal concentration of Hcy-thiolactone is obtained. Modeling Indicators Molecular changes: Caused microglia activation in the retina. Increased indicators: pro-inflammatory cytokines IP-10, TNFα, IL-1β, and IL6 Correlated Product(s): L-Homocysteine (HY-W010347) Opposite Product(s): /

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References：

[1]. Jakubowski H, et al. Homocysteine thiolactone: metabolic origin and protein homocysteinylation in humans. J Nutr. 2000 Feb;130(2S Suppl):377S-381S.  [Content Brief]

[2]. Nehal M Elsherbiny, et al. Homocysteine Induces Inflammation in Retina and Brain. Biomolecules. 2020 Mar 3;10(3):393.  [Content Brief]

[3]. Ahmed S Ibrahim, et al. Hyperhomocysteinemia disrupts retinal pigment epithelial structure and function with features of age-related macular degeneration. Oncotarget. 2016 Feb 23;7(8):8532-45.  [Content Brief]

[4]. Olivera Stanojlović, et al. Two types of seizures in homocysteine thiolactone-treated adult rats, behavioral and electroencephalographic study. Cell Mol Neurobiol. 2009 May;29(3):329-39.  [Content Brief]