MoBiTec - Model HIST01 -Anti-HIS-Tag Antibody
Vectors for expression of fusion proteins possessing a (His)6-Tag are commonly used for simple purification of recombinant proteins by metal affinity chromatography (e.g. nickel-nitrilotriacetic acid). The MoBiTec His-Tag antibody is very useful in monitoring (His)6-Tag fusion protein expression and purification and shows a markedly increased sensitivity for (His)6-Tags compared with other commercially available anti-His-Tag antibodies.
Antigen
KLH-Gly-Ser-(His)6
Source
Rabbit, polyclonal
Contents
The antibody is supplied as 100 µl serum.
Specificity
C- or N-terminal (His)6–Tag
Recommended Dilution
The anti-His-Tag antibody can be used for Western blotting at a 1:1,000 dilution.
Immunoprecipitation and other immunological applications have not been tested so far.
References
Porath, J. (1992) Immobilized metal ion affinity chromatography. Protein Express. Purif. 3, 263-281.