Electrospinning - Model MCF7 -Breast Cancer Cells

The Mimetix fibres are labelled with Rhodamine 6G. The thickness of Mimetix scaffold allows for having a 3D environment while maintaining a certain translucence, thus allowing for easy and robust imaging. This study has investigated the localisation depth of MCF7 breast cancer cells in the Mimetix scaffold after 4 days of culture (nuclei stained with TO-PRO-3, blue). The video below shows a Z-stack (slices through the scaffold from the top to the bottom at 2 μm steps), 4 days after seeding. At day 1 most cells are located with the top 1/3 of the scaffold, whereas they occupy the top 2/3 of the scaffold with few cells observed even deeper at day 4.