TestLine Clinical Diagnostics s.r.o
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- Model DNA096 - Elisa Kits EIA dsDNA
Model DNA096 -Elisa Kits EIA dsDNA
Enzyme immunoassay for the determination of IgG antibodies to dsDNA antigen in human serum or plasma. Microtitre wells are coated with highly purified native antigen dsDNA. If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete). The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.
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Advantages:
- The total assay time is about 1 hour 30 min.
- High sensitivity and specificity of the test.
- Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (10, 50, 200, 600 IU/ml).
- Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in IU/ml.
- Ready-to-use, color-coded components.
- Single-component substrate.
- Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).
Application:
Differential diagnostics of systemic autoimmune diseases.
Brief assay procedure:
- Dilute samples (1:101).
- Pipette Controls and diluted Samples.
- Incubate at 37°C for 30 min.
- Aspirate and wash the wells 5×
- Pipette Conjugate.
- Incubate at 37°C for 30 min.
- Aspirate and wash the wells 5×
- Pipette Substrate (TMB-Complete).
- Incubate at 37°C for 15 min.
- Pipette Stop Solution.
- Read color intensity at 450 nm.
- Evaluate the test.