Twist - Model Anti-ACE2 -Human Antibodies

Kinetic Data

The anti-ACE2 antibodies exhibit very high specificity and affinity to their antigen targets with affinities in the picomolar to nanomolar range. The table below lists Fc-capture and direct capture (DC) data from this assay.

Cell Binding Data 

The entire panel shows varying degrees of specific binding to VERO E6 cells as shown in the table below, and an example of the flow cytometry data for a single clone is also shown. S1 Fc fusion protein and S1 RBD Fc fusion (expressed in-house) were added as positive controls for ACE2 binding.

Competition Assay
 
To assess each antibody clone’s ability to interfere with the binding of the S1 protein to ACE2, serial dilutions of ACE2 were added to biotinylated ACE2 protein. The mixture was then added to SARS-CoV-2 S protein RBD immobilized on an ELISA plate and detected with streptavidin-HRP. Background was subtracted from data points prior to log transformation and curve fitting. IC50 values for all antibody clones are presented in the table.

• Description Individual antibody clones that recognize Human ACE2
• Host Species Human
• Species Reactivity Reacts with Human ACE2
• Panning Antigen Recombinant fragment Human ACE2 ECD
• Gene ID 59272
• Database NM_001371415
• Clonality eecombinant Monoclonal
• Isotype IgG antibodies are IgG2 and VHH Fc antibodies are IgG1
• Purity >95% by CE-SDS
• Plate Format Volume 20 µL/well, Concentration 1 mg/mL
• Tube Format Volume 100 μL/tube, Concentration 1 mg/mL