Human Premature Ovarian Failure Protein 1B (POF1B) ELISA Kit

• Product name: Human Premature Ovarian Failure Protein 1B (POF1B) ELISA Kit
• Method: Sandwich
• Synonyms: 
• Detection range: 0.312-20ng/mL
• Target Protein: POF1B
• Size: 96T/48T
• Quality guarantee period: for 12 months, 16 months

The microtiter plate provided in this kit has been pre-coated with an antibody specific to POF1B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to POF1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain POF1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of POF1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.