- Home
- Companies
- Fluxion Biosciences Inc
- Products
- Spotlight - Model 59 - Oncology Panel
Spotlight - Model 59 -Oncology Panel
Designed specifically for detection of somatic mutations fromliquid biopsy samples, the Spotlight 59 Oncology Panel is aturnkeysolution for Illumina sequencers, offeringultra-sensitive variant detectionfrom low input quantities of DNA. The high sensitivity of Spotlight 59makes it ideal for cfDNA, CTCs, andother challenging sample types. Combined with ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight 59 delivers >90% sensitivity and % allele frequency.CompatiblewithcfDNA and CTCs fromFluxion’s IsoFlux Liquid Biopsy System, Spotlight 59 creates a complete sample-to-answer assay for high sensitivity mutation detectionof solid tumor cancersfrom blood samples
The Spotlight 59 liquid biopsy workflow for ultra-accurate detection of rare variants starts with isolation of DNA from a blood sample. This is followed by targeted amplification andsequencing at 15,000Xper sample(standard protocol). Fluxion’s ERASE-Seq bioinformatics solution is accessedvia secure upload to aHIPAA-compliant cloud-based server. Statistical comparison to a well-characterizednormal control specific to the Spotlight 59 panel is performed, allowingaccurate variant calling to 0.1% allele frequencyand below. Variant calls are available for download within 24-48hours. The analysis service is includedfor Spotlight 59 customers.
