Transcreener® ADP² - Kinase Assay Kits
Transcreener assays are competitive immunoassays in a mix-and-read format. Displacement of tracer from the ADP antibody causes a change in its fluorescence properties. Transcreener is the simplest, most direct method for measuring ADP formed during a kinase reaction. Since the introduction of the first product in 2006, Transcreener has been used in millions of wells for HTS and lead discovery.
Overcome the need for time-consuming, one-off development for individual members within an enzyme family by using a single set of reagents that detect the invariant product – ADP. The assay can be used with virtually any substrate including peptide substrates, protein substrates, and lipid substrates.
Example enzyme targets include:
- Protein kinases
- Lipid kinases
- Carbohydrate kinases
- ATPases
- Chaperonins (Hsp90, etc.)
- Nucleotidases
- Carboxylases
- Helicases
Learn how Eli Lilly used Transcreener to discovery and patent Aurora A Kinase Inhibitors for cancer therapeutic development.
See how researchers at the University of Wisconsin - Milwaukee used the ADP assay to measure helicase activity in an effort to find small molecule inhibitors as a therapeutic for hepatitis C.
Discover how Pfizer used the assay to find Acetyl-CoA Carboxylase Inhibitors as potential treatments for a variety of indications including diabetes and metabolic syndrome.
- Screen for small molecule inhibitors
- Determine enzymatic activity
- Profile inhibitor potency/determine IC50 values
- Measure residence time/off-rate/dissociation of lead molecules with target
- Inhibitor selectivity profiling
- Mechanism of action studies
- Simple homogenous biochemical assay
- Direct detection of ADP eliminates laborious coupling steps
- Non-radioactive, safe assay method
- HTS Ready – Use in 96-well, 384-well, or 1536-well formats
- Choose from Fluorescent Polarization (FP), Fluorescent Intensity (FI), or time-resolved Förster-resonance-energy-transfer (TR-FRET) formats
- Sensitive, robust detection of ADP. This means up to 10X less enzyme, a significant cost reduction on large screens.
- Use with ATP concentrations from 0.1 to 1000 µM
- Far-red tracer further minimizes interference from fluorescent compounds and light scattering