Nippon FastGene Block & Go - Model FG-CH05 -Western Blocking Solution and Signal Enhancer

• 2-in-1 solution: Blocking reagent and signal enhancer
• 3-in-1 step procedure: Blocking of the membrane + primary antibody + secondary antibody in one step
• Time-saving: Up to 2.5 h less hands-on-time compared to conventional protocol using milk or BSA
• Increased detection sensitivity: Detection of proteins from up to 0.5 µg lysate
• Flexible use: Usable in single step or two step protocols
• Protein-free: Reduced overall background and minimization of non-specific signals

• 3 steps in one: blocking of the membrane, primary and secondary antibody in one step
• Enhanced antibody signal: 2-5-fold increase in signal intensity for most protein targets.
• Enhance time-saving: It saves at least 2 hours in the antibody detection process during the Western Blot, with only one hour needed.
• Effective with any ECL: After the antibody detection process, the signal can be developed with both HRP (horseradish peroxidase) and AP (alkaline phosphatase) substrates.
• Less hands-on steps: No 3 wash steps are required, meaning no need to transfer the membrane in&out of the container.
• Compatible with PVDF & NC membrane: Regardless of the pore size, ‘Block & Go’ minimizes the background from non-specific protein binding by antibodies.
• Protein free: Reduces overall background and minimizes non-specific signals often seen with ECL detection.

By combining 3 steps in one step (blocking + primary antibody + secondary antibody), the procedure can be reduced about 1 h (2-step procedure) or even 2.5 h (1-step procedure) compared to conventional protocol using milk or BSA.