Conformation and Size Variants Service
In addition to self-association behavior (e.g. desired oligomerization), the conformational status of proteins and other biomolecules (e.g. denaturation, misfolded conformers) is crucial for understanding molecular functions and evaluate stability/safety/efficacy issues of drug products. Therefore, discriminating between size and conformation variants is an ubiquitous task in characterizing the size distribution of biomolecules in solution.
Nanolytics offers the most advanced instruments combined with an extensive experience in data evaluation, in order to differentiate conformation- and size variants using various AUC techniques. As alteration in conformation (i.e. the shape) of the analyte results in distinct frictional properties, application of specific data algorithms for evaluating sedimentation velocity experiments is an essential tool. Of particular relevance are data fits generating 2D sedimentation coefficient distributions assigning a pair of distinct sedimentation coefficient and frictional ratio to each sedimenting population.