How to Reconstitute Lyophilized Proteins - Video

Leadgene Lyophilized Protein Reconstitution Protocol
1. Unopened ampoules can be stored at -20°C or -80°C.
2. Need not spin the ampoule. Open the cap carefully, then dissolve the lyophilized protein with sterile water for a 100 μg/mL concentration or according to the product's Certificate of Analysis (CoA). (Note: Need not add carrier* to the initial reconstitution solution).
3. Rinse the inner side of the ampoule gently and stand for at least 20 minutes at room temperature. (Important Note: Do Not Vortex!)
4. Use the reconstituted solution immediately or store it by distributing it to aliquots and store at -20 °C. (Important Note: Avoid repeated freeze-thaw cycles)
5. We recommend diluting the solution to working concentration by using buffers or medium containing a carrier*. (Important Note: Do not dilute the working solution with water, which may cause protein degradation.) *Carriers: 0.1% BSA or 5% HSA or 10% FBS.


偉喬生醫 凍乾蛋白複溶步驟：
1. 重組蛋白在未開封情況下可保存於-20°C或-80°C。
2. 回溶前無須將安瓶離心，只需小心打開安瓶。將滅菌水小心加入安瓶，使蛋白濃度成為100 μg/mL，也可參考Certificate of Analysis (CoA)建議加入之體積。(注意：初始回溶時不建議使用緩衝液。)
3. 小心沖洗管壁，盡量使附著於管內之蛋白皆回溶。室溫下靜置20分鐘。(特別注意：不可劇烈搖晃瓶身。)
4. 回溶後之蛋白可立刻使用或分裝凍於-20 °C，避免重複冷凍解凍。
5. 若需要進一步稀釋使用，建議使用含有0.1% BSA、5% HAS或10% FBS之緩衝液進行稀釋。