Gene Editing - Medical / Health Care
Isolating edited-pluripotent stem cells with traditional sorting techniques can subject the cells to rough conditions in which gene expression, genomic stability and overall cell-health can become compromised. Microfluidic cell sorting with the WOLF, along with single cell deposition using the N1 single cell dispenser, eliminates the challenges that pluripotent stem cells face by sorting at gentle pressures without decompression shock and shear stress, and allow for efficient single cell deposition for high monoclonal outgrowth. Learn more about how cell sorting can improve workflows such as: CRISPR and disease modelling, Protection of Karyotype, Generation of Genome-Edited iPSCs.
Genome engineering using CRISPR/Cas9 has transformed the way scientific biological research has progressed over the years. However, the protocols for isolating edited-pluripotent stem cells subject those cells to rough conditions in which gene expression, genomic stability and overall cell-health can become compromised. These issues are compounded additionally with intrinsic challenges in culturing single cells for monoclonal outgrowth.
Microfluidic cell sorting with the WOLF®, along with single cell deposition using the N1 single cell dispenser, eliminates the challenges that pluripotent stem cells face by sorting at gentle pressures without decompression shock and shear stress, and allow for efficient single cell deposition for high monoclonal outgrowth. This improved workflow will allow for higher throughput pluripotent stem cell research, which is valuable for both basic and clinical research.
Efficient antibody screening by sorting into a 96- or 384-well plate.
Researchers can effortlessly deposit single cell iPSCs into various types of 96- or 384-well plates for maximum cell line development flexibility.
Eliminate carryover and instrument contamination through disposable microfluidic cartridges.
When working with multiple iPSC cell lines, it can be critical to ensure that they do not contaminate each other. Sample carryover is eliminated with the WOLF’s 100% disposable cartridges.
Gentle cell sorting leads to high clonal outgrowth.
Induced pluripotent stem cells are particularly prone to issues of genomic instability. Scientists can be assured that sorting will have little to no effect on cell integrity when using the WOLF’s gentle <2 psi pressure microfluidic sorting cartridges.
Simple sorting to identify high producing clones.
There is no need to be a flow cytometry expert when completing cell sorting on the WOLF. The straightforward software allows users to work independently shortly after training.
Compact design to fit in a biosafety cabinet.
Many laboratories do not have space for a large, conventional cell sorter. Save on space by implementing the WOLF into a cell line development workflow, at only 2 cubic feet. The compact design fits in biosafety cabinet for sterile cell sorting.
As fast as 3 minutes for a 96-well plate and 9 minutes for a 384-well plate.
Researchers can spend more time completing other experiments when plating single iPSC cells takes as little as 3-9 minutes per 96- or 384-well plate.
Induced pluripotent stem cells provide considerable advantages when it comes to in vitro modelling of human diseases. The ability for iPSCs to be reprogrammed and differentiate into virtually any cell type will allow for patient-specific disease models. This holds a powerful promise to perform high throughput drug screening, and other testing of therapeutics. The WOLF has been used to isolate healthy populations of Autism and Alzheimer’s iPSC CRISPR/Cas9 edited cell lines for disease modelling. The viability and proliferation of these fragile cell lines fared significantly better than those that were sorted on a conventional electrostatic sorter. The challenge of low proliferation and low viability can be overcome using a microfluidic sorter like the WOLF in sorting fluorescent CRISPR/Cas9 edited iPSC cell lines.