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Genomics - Medical / Health Care
Sample preparation is a significant yet underappreciated step that allows for high quality sequencing and analysis, critical to achieving breakthroughs in medicine, agriculture, and other industries. NanoCellect’s microfluidic cell sorter, the WOLF, allow the cell’s integrity to be maintained for numerous sequencing and multi-omics techniques. The WOLF’s ability to flexibly fit into any genomic researcher’s lab, make it a key step to any cell sequencing analysis workflow. 10x Genomics RNA-seq library prep, Single-Cell sorting on PCR plate, Whole-genome sequencing methods
With over 40 trillion cells in the human body and hundreds of cell types, cells have nevertheless traditionally been studied using bulk methods at the cell population or tissue level rather than single-cell level. However, the field of genomics and computational biology has progressed immensely over the last decade and new single-cell genomics approaches and techniques are advancing our understanding of human disease and many other biological problems. Following the emergence of next-generation sequencing (NGS), high-throughput genomics and transcriptomics measurements using DNA and RNA sequencing developed rapidly. Single-cell RNA sequencing (RNA-Seq) has also provided new insight into gene expression and epigenetic mechanisms behind important biological processes.
Sample preparation is a significant yet underappreciated step that allows for high-quality sequencing and single-cell analysis, which is critical to achieving breakthroughs in medicine, agriculture, and other industries.
NanoCellect’s microfluidic cell sorter, the WOLF®, allows the cell’s integrity to be maintained for numerous sequencing and multi-omics techniques. The WOLF’s ability to flexibly fit into any genomic researcher’s lab makes it a key step to any cell suspension genomic data analysis and cell sequencing analysis workflow.
- Simple sorting to optimize sequencing data.
Even if genomic researchers are flow cytometry beginners, the WOLF has an intuitive and simple software workflow. It takes a day of training before novice users are up and running in their own lab.
- Clean debris and doublets to save on sequencing reagents.
The WOLF provides a quick solution for cleaning up samples prior to genomic studies. Scientists can be assured they will save money on reagent cost because reads are not wasted on dead cells and debris, which are eliminated on the WOLF.
- Happy cells lead to clearer biological insight.
Avoid stressing cells prior to transcriptomic studies. Researchers can be confident that, by utilizing a gentle sort upstream of library prep and sequencing, the readout will be unaffected by this sample prep process. Good quality sample prep up front, leads to clearer biological insight.
- Compact benchtop sorting ahead of library prep.
Many laboratories do not have space for a large, conventional cell sorter. Save on space by implementing the WOLF into a sequencing workflow, at only 2 cubic feet.
- Eliminate carryover and instrument contamination with disposable microfluidic cartridges.
Researchers often need to sort many different cell types, and sample cross-contamination can be a huge issue while sequencing. The WOLF utilizes 100% disposable microfluidic cartridges and eliminates the issue altogether.
- Save time on sample prep by sorting at the bench.
Researchers can use the WOLF when it is convenient for them, at any time, to get the right cells for the best quality sequencing data.