Single-cell omics with Live-seq - Medical / Health Care - Clinical Services
The novel non-invasive method for single-cell omics
The novel method that keeps your cells alive during biopsy from single cells
Single-cell omics addresses cellular heterogeneity head on by isolating and analyzing molecular components from individual cells.
While many of the existing technologies rely on cell lysis — single-cell biopsy is the only non-invasive single-cell analysis method that allows researchers to take transcriptome snapshots of a cell without killing it.
Gene expression profiles obtained from single-cell biopsies are suitable representations of lysed cell transcriptomes. (Chen et al., 2021)
Non-invasive single-cell biopsy
- Get reliable results without altering gene expression, cell phenotype, or cell-cell interactions.
Continuous and live-monitoring of a single-cell with biopsies
- FluidFM extraction preserves cell viability: extract several times from the same cell within the same run or periodically over time.
Analyze temporal gene expression
- Sequential profiling of the single-cell’s transcriptome
Live-seq and the single-cell biopsy method brings a new paradigm to single-cell transcriptomics by enabling researchers to take transcriptome snapshots of a cell without killing it. The protocol is so straight-forward and easy that new lab members can perform it whether it is for single-cell extraction or single-cell injection.
- Record a cell transcriptome prior to phenotyping.
- Record transcriptional events over time to reveal how molecular components influence cell behavior.
- Directly link an individual cell’s history and trajectory to unravel past cell states and understand lineage decisions.
- Take a biopsy from cells of a heterogeneous disease before and after exposure to a specific therapeutic to identify molecular signatures for early drug development.
Powered by FluidFM technology + a highly sensitive low-input RNA-seq protocol
The Live-seq method pairs the FluidFM OMNIUM system with a highly sensitive low-input RNA-seq protocol, which stems from a collaboration between ETH Zurich and EPFL. The FluidFM OMNIUM allows you to extract sub-picoliter volumes from cellular compartments of a living single cell and then isolate the extract for further analysis. By avoiding destructive methods, such as cell lysis, you can perform further downstream molecular and phenotypic analysis and even transcriptome profiling over time on the very same cell. This introduces trajectory instead of end-point analysis to your transcriptomics, metabolomics, proteomics, or any other omics studies.
- Semi-automated application-specific workflows and probe handling.
- Easy-to-use, point & click interfaces.
- Intuitive software with easy cell selection – point-and-click to target a specific cell.
- Optimized probes.
- Hollow, force-sensitive probes particularly designed for specific applications.