In Vivo Biotin Recombinant Antibodies

Yeast produces biotinylated recombinant antibodies in vivo. Researchers have now developed a new method to generate in vivo biotinylated recombinant antibodies secreted by yeast. In vivo biotinylation supports efficient biotinylation for accurate testing. Yeast is a single-celled fungal organism that has played an important role in the field of biogenetics in recent years. Based on the expansion system of the yeast gene codon, it can exploratoryly use site-directed co-translational modification as a research method for synthetic epigenetics. Through the functionalized introduction group, co-translation can be realized to modify and maintain the life activity of yeast. In-depth research on yeast can provide efficient ideas and perspectives in the long modification process. Yeast is an important tool for genetic research. Knocking out genes in it can realize the processing of genetic modification.

Utilizing the important role of yeast in genetic research, the research of biotinylated recombinant protein antibody in vivo can be realized. 

What are recombinant antibodies?
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination and are produced by immunizing animals with an antigen to elicit an immune response. Polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, whereas monoclonal antibodies are derived from a single B cell clone and are specific for only a single epitope. Recombinant antibodies are monoclonal antibodies. The antibody gene is cloned into an expression vector, which is transfected into a suitable host cell line for antibody expression. Mammalian, bacterial, yeast, and insect cell lines are all suitable for recombinant antibody production.

Preparation method of biotinylated antibody
Active biotin derivatives bind to functional groups on protein surfaces
Site-specific biotinylation of recombinant antibodies
Recombinant antibody: no animal origin
Production of recombinant antibodies avoids the use of animals, and recombinant antibodies are purified from TCS transfected host cell lines