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Gene Expression Articles & Analysis: This-Year
15 articles found
Techniques such as immunohistochemistry and in situ hybridization can be performed on these sections to study protein expression and gene activity, respectively. This molecular insight is crucial for understanding the pathophysiological processes underlying kidney diseases and for the development of targeted therapies. ...
Utilizing FFPE blocks derived from the rat thymus enables researchers to conduct various assays, including immunohistochemistry, in situ hybridization, and gene expression studies. These methods offer insights into the thymus’s cellular composition and functionality. ...
The Sweeping Functional Roles of Acetylation The "acetylation code" has profound functional consequences throughout the cell. The classic example is in gene expression. Histone proteins, which package DNA into chromatin, are rich in lysine residues. ...
These features resemble those of ESCs, indicating the reprogrammed cells have regained pluripotency. Pluripotency Markers The expression of specific pluripotency markers is another critical aspect of iPSC characterization. Stem cell surface markers such as SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 are routinely analyzed via flow cytometry or immunocytochemistry. Additionally, the ...
The Power of Optogenetics Optogenetics enables researchers to control specific neurons using light, offering insights into neural circuits’ dynamics and behaviors. By introducing genes that encode for light-activated ion channels or pumps into specific populations of neurons, scientists can activate or inhibit neuronal activity in real-time. ...
Moreover, lentiviral vectors can accommodate large genetic payloads, enabling the delivery of the Cas9 protein, guide RNA, and additional elements necessary for advanced gene-editing strategies. Their ability to integrate into the host genome ensures long-lasting effects, making them a preferred choice for stable gene expression. ...
Stress response mechanisms: Differential expression of heat shock proteins, particularly GroEL and DnaK chaperones, correlates with viability post-lyophilization—a critical consideration when selecting freeze-dried powder formulations for research. ...
Tumors often exhibit heterogeneous genetic and protein expression profiles, which can influence prognosis and treatment decisions. By employing ImmunoFISH, researchers can simultaneously assess the expression of specific proteins and the corresponding genetic changes within the same tissue section. ...
This technique is particularly advantageous for those seeking to identify and map gene expression patterns within tissues, serving as a foundational method in many research applications. ...
Immunohistochemistry: Frozen sections enable the study of protein expression within lung tissues. Specific antibodies can be used to target and visualize proteins associated with inflammatory responses, fibrosis, and cellular proliferation. Molecular Studies: Techniques such as in situ hybridization can be performed on frozen sections to investigate gene ...
They possess a high transfection efficiency, making them ideal for gene expression studies, protein production, and functional assays. The use of HEK293T as the background for the Monkey DLL3 stable cell line enhances the ability to manipulate the genetic material effectively, enabling the expression of DLL3 in a controlled manner. Development of ...
Simple Summary The present study investigated possible administration of pectin derived from apple pomace as a beneficial and cost-effective feed additive to be used in trout culture. To achieve the aim, a range of parameters were measured including immune parameters (both at physiological and molecular levels), growth performance and digestive enzyme activity. The results showed promising ...
Furthermore, exosomes also carry small RNAs, such as microRNAs, which influence gene expression and can modulate metabolic pathways in recipient cells. ...
They play a critical role in RNA interference (RNAi), a biological process used by cells to regulate gene expression and defend against viral infections. The ability to design dsRNA specific to target genes has opened up new avenues for gene silencing technology. ...
Nuclear receptors are a class of proteins that play a vital role in cellular communication, gene expression, and overall homeostasis within the body. They act as transcription factors, responding to various stimuli, including hormones, nutrients, and even environmental agents. ...