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Next Generation Sequencing Articles & Analysis: This-Year

4 articles found

A Comprehensive Guide to sgRNA Design and Confirmation

A Comprehensive Guide to sgRNA Design and Confirmation

Target Selection: The initial step is identifying the genomic sequence to be edited. This involves analyzing the target gene’s sequence and selecting a region that is critical for the desired modification. ...

ByCreative Biogene


What is the Role of tRNA Methylation Modification in the Cell Cycle?

What is the Role of tRNA Methylation Modification in the Cell Cycle?

Post-transcriptional modifications of RNA molecules include methylation, acetylation deamination, isomerization, glycosylation, thiolation and pseudouracil modification among other types. Methylation represents the predominant post-transcriptional modification found within transfer RNA (tRNA) molecules. tRNA methylation has maintained its evolutionary conservation while also exhibiting dynamic ...

ByCreative Diagnostics


Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications   

Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications  

For metagenomic analysis, the moderate cutting frequency of HindII often produces fragment sizes ideally suited for next-generation sequencing library preparation, typically ranging from 2-10kb depending on GC content. ...

ByCreative Enzymes


Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications   

Optimizing HindII Digestion Protocols for Challenging DNA Templates in Advanced Molecular Biology Applications  

For metagenomic analysis, the moderate cutting frequency of HindII often produces fragment sizes ideally suited for next-generation sequencing library preparation, typically ranging from 2-10kb depending on GC content. ...

ByCreative Enzymes

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