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In the development of new drugs high throughput screening (HTS) is often used to screen large libraries of compounds for inhibitors or stimulators of various reactions. The firefly luciferase reaction can be performed reliably in a small reaction volume and is therefore highly suitable for HTS. Our Protein Kinase Kit RR can be used to measure not only protein kinase, but also all ATPases and aminoacyl tRNA synthetases. Growth inhibition from e.g. antibiotics can be measured. Gene expression can be monitored. Please have a look at the application listing below to navigate to the product that matches your need.
Clinical diagnostics as e.g. measurement of bacteria in urine with a luciferase based assay of bacterial ATP can be used in the diagnosis of urinary tract infection (UTI). Screening for Duchenne’s Muscular Dystrophy (DMD) has been performed in 528,410 newborn boys in a German study. Luciferase based assays of ATP and ATP+AMP is more and more used to control hospital hygiene. Please have a look at the application listing below to navigate to the product that matches your need.
Methodology: The luc gene coding for firefly luciferase may be attached to another gene of interest. The expressing of this gene may then be estimated from the level of luciferase expression measured as light. This may be done in vitro, i.e. after lysing the cells adding ATP and D-luciferin, or in vivo. In the latter case D-luciferin has to enter the cells. Even whole live animals or plants may be studied, “in vivo imaging”, by injecting D-luciferin into the organism (ATP is already in the cells).