Creative Bioarray services

creative bioarray - Alkaline Phosphatase Staining

Alkaline phosphatase (AP) staining is a rapid method for screening pluripotent cells by using AP activity. AP is a hydrolase responsible for the dephosphorylation of many molecules, such as nucleotides, proteins, and alkaloids under alkaline conditions. AP activity is higher in pluripotent cells but is greatly decreased in more differentiated cell types. The technique described herein may be used to enumerate pluripotent cells during differentiation in the presence or absence of specific genetic manipulations or small chemical modulators. It may also be used to monitor induced pluripotency using defined factors from more differentiated cell types.

creative bioarray - Pericytes

Pericytes are vascular mural cells embedded in the basement membrane of blood microvessels. They extend their processes along capillaries, pre-capillary arterioles and post-capillary venules. Pericytes regulate blood-brain barrier (BBB) permeability, angiogenesis, clearance, cerebral blood flow (CBF), neuroinflammation and stem cell activity.

creative bioarray - Pooled Human Hepatocytes

Pooled Human Hepatocytes are the chief functional cells of the liver, responsible for the selective uptake, metabolism and excretion of most drugs. Cells for use in plate cultures or suspension assays represent a key tool for predicting properties such as hepatotoxicity, metabolism, and potential drug-drug interactions. Since these cells mimic the properties of the liver, researchers can evaluate the metabolism, drug-drug interaction and toxicity of drug candidates in a cost-effective manner.

creative bioarray - Establishment and Culture Protocol of mESCs Cell Lines

Embryonic stem cells (ESCs) are abbreviated as ES, EK, or ESC cells. They are a class of cells isolated from early embryos (before the proto-intestinal stage) or primitive gonads, which have the properties of unlimited proliferation, self-renewal and multidirectional differentiation in vitro in culture. ES cells can be induced to differentiate into almost all cell types of the organism, both in vitro and in vivo environments.

ADME and Pharmacokinetic Services

Creative Bioarray provides various in vitro ADME/PK services including high-throughput ADME screening, in vitro metabolism, in vitro permeability and transporter assays. ADME is short for "absorption, distribution, metabolism, and excretion". The four properties determine the drug level within a body, the drug exposure to tissues, and the metabolic process of a compound, which can help predict the bioavailability and bioactivity of a drug-whether a drug can reach its intended target and confer a therapeutic effect. Therefore, understanding the ADME properties of the compounds is crucial to the drug developing process.