MtoZ Biolabs articles

Persistent histone phosphorylation refers to the continuous or long-term phosphorylation of specific amino acid residues (usually serine or threonine) on histones under certain cellular conditions or stimuli. This phosphorylation is usually catalyzed by specific kinases (such as protein kinase A, protein kinase C, or MAP kinase) and can be reversed by phosphatases. Persistent histone phosphorylation plays a crucial role in the regulation of gene expression, cell cycle progression, DNA repair,

Post-translational modifications (PTMs) of histones are crucial for protein structure regulation, playing key roles in gene expression regulation, DNA repair, cell signaling, and more. These PTMs of histones can be achieved by adding or removing different chemical groups or proteins, thus affecting chromatin structure and function. Here are some common types of histone PTMs.

1. Acetylation

The addition of acetyl groups usually occurs on lysine res

Amino acid sequence analysis is a fundamental field in protein research, involving the identification and interpretation of the linear sequence of amino acids in a protein. The amino acid sequence of a protein can provide important information on its structure, function, and evolution. Here are the applications of amino acid sequence analysis in protein research.

Sequence Acquisition

1. Determining the Amino Acid Sequence

Ubiquitination detection of proteins is a biochemical technique used to identify and analyze ubiquitination modifications on proteins. Ubiquitination is a post-translational modification process that involves the covalent linkage of the small protein ubiquitin to lysine residues of a target protein, a modification that plays a crucial role in the regulation of protein degradation, signal transduction, cell cycle control, and more.

Since the discovery of ubiquitin in the 197

The feasibility of using blood samples for histone modification research is evident. Blood contains various cell types, including red blood cells, white blood cells, and platelets. For histone modification research, the focus is usually on white blood cells because they contain cell nuclei, and histones are found within the nucleus.

Analysis Workflow

1. Sample Selection and Treatment

Blood samples need t