RB-LCFC - Data Sheet

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol, forming cholesteryl ester. This enzymatic activity can occur on the surface of high density lipoprotein (HDL) and the cholesteryl esters formed by LCAT may be packed into the core of HDL. The Roar LCFC-LCAT Activity Assay Kit is a homogeneous fluorometric assay useful for measuring the acyltransferase activity of LCAT by non-enzymatic means. This assay is, therefore, not affected by iodoacetate or other LCAT inhibitors. 1 The method uses a reagent designed for detecting changes in free cholesterol concentration without cholesterol oxidase, peroxidase or the generation of hydrogen peroxide.