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MedChemExpress - Model Streptozotocin - 18883-66-4

Streptozotocin (Streptozocin; STZ) is an antibiotic widely used in experimental animal models of induced diabetes. Streptozotocin enters B cells via the glucose transporter (GLUT2) and causes the alkylation of DNA ( DNA-methylating ). Streptozotocin can induce the apoptosis of β cells[1][2][3].

Streptozotocin

MCE China：Streptozotocin

Brand：MedChemExpress (MCE)

Cat. No.HY-13753

CAS：18883-66-4

Synonyms：Streptozocin; NSC-85998; U 9889

Purity：99.15%

Storage：-20°C, sealed storage, away from moisture and light *The compound is unstable in solutions, freshly prepared is recommended.

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Streptozotocin (Streptozocin; STZ) is an antibiotic widely used in experimental animal models of induced diabetes. Streptozotocin enters B cells via the glucose transporter (GLUT2) and causes the alkylation of DNA ( DNA-methylating ). Streptozotocin can induce the apoptosis of β cells.

In Vitro：The IC50 values of Streptozotocin for HL60, K562 and C1498 cells were 11.7, 904 and 1024 μg/ml, respectively[3].

In Vivo：Streptozotocin is suitable for constructing models of type 1 and type 2 diabetes. Highly water-soluble, Streptozotocin, once absorbed, distributes widely throughout the body, including crossing the blood-brain barrier and placenta, entering various tissues. In the liver, Streptozotocin undergoes chemical modification, converting into an active form that causes DNA methylation and damages pancreatic β-cells, leading to diabetes. The elimination half-life of Streptozotocin varies depending on the species and route of administration. .f12{ font-size: 12px; } .fwb{ font-weight: bold; } .lh22{ line-height: 22px;; } .lh23 { line-height: 23px; } .pl13{ padding-left: 13px;; } .part { margin-top: 18px; } .mold-first-tit { width: 100%; height: 44px; line-height: 44px; background: #F9F7FB; border-bottom: 1px solid #EBE4F6; padding-left: 16px; box-sizing: border-box; margin-bottom: 17px; } .mold-second-tit:before { content:""; width: 6px; height: 6px; display: inline-block; border-radius: 50%; background: rgba(255,102,0,0.4); margin-right: 12px; position: relative; top: -3px; } .lft-border { border-left: 1px dotted #EBE4F6; padding-right: 12px; margin-left: 3px; box-sizing: border-box; padding-bottom: 12px; } /* .part .dec:last-child { border-bottom: 0; } */ .dec { margin: 10px 15px 0; padding-bottom: 10px; border-bottom: 1px dashed #EBE4F6; } .btm-border { border-left: 1px dashed #EBE4F6; } .text-bg { margin-top: 10px; background: #FFFBF1; padding: 14px; border-bottom: 0; position: relative; } .text-note-bg { margin-top: 10px; background: #FFFDF7; padding: 12px; border-bottom: 0; position: relative; } .text-note { width: 51px; height: 20px; line-height: 20px; background: #FFE2AA; text-align: center; border-radius: 0 0 8px 0; position: absolute; top: 0; left: 0; } .text-note-dec { margin-top: 15px;; } Induction of Type 1 Diabetes Mellitus (T1DM)[3][4][5] Background Induces disease by direct destroying the animal's islet β beta cells. Specific Mmodeling Methods Mice: C57BL/6 • female • 10 week-oldAdministration: 200 mg/kg • i.p. • single high dose.Rat: Sprague-Dawley or Wistar rats • male • 8-10 weeks-oldAdministration: 65 mg/kg • i.p. • single high dose. Note Tips: 1) The sensitivity of different species of animals to STZ varies greatly, and it is recommended to use male rats (female mice are more tolerant to STZ) [3];2) Fasting without water before administration can increase the sensitivity of pancreatic β cells to STZ. STZ injection in model animals generally requires rapid injection;3) Different strains of mice have different sensitivities to STZ. Studies have reported that the DBA/2 strain is the most sensitive, followed by C57BL6. Balb/cJ mice are resistant to multiple low-dose STZ-induced diabetes[4];4) After STZ treatment, animals die due to fatal hypoglycemia due to massive necrosis of pancreatic β-cells and sudden release of insulin, usually within 48 hours after injection. To prevent this, it is best to provide animals with 10% sucrose water regularly after STZ treatment. If animal mortality exceeds 20% when using a single high-dose STZ diabetic mouse protocol, treat animals with an intraperitoneal injection of 5% glucose solution within 6 hours of STZ injection[5];5) Preliminary experiments are required, and it is not recommended to directly use the administration methods and dosages in the literature. Modeling Indicators Blood glucose level : Blood glucose level exceeds 300 mg/dL(16.7 mmoL/L). Other indicators : generally accompanied by increased water intake, urine volume, and weight loss. Serum biochemical indexes such as total cholesterol, aspartate aminotransferase, triglyceride and low density lipoprotein also increased significantly with the occurrence of diabetes. Correlated Product(s): / Opposite Product(s): / .f12{ font-size: 12px; } .fwb{ font-weight: bold; } .lh22{ line-height: 22px;; } .lh23 { line-height: 23px; } .pl13{ padding-left: 13px;; } .part { margin-top: 18px; } .mold-first-tit { width: 100%; height: 44px; line-height: 44px; background: #F9F7FB; border-bottom: 1px solid #EBE4F6; padding-left: 16px; box-sizing: border-box; margin-bottom: 17px; } .mold-second-tit:before { content:""; width: 6px; height: 6px; display: inline-block; border-radius: 50%; background: rgba(255,102,0,0.4); margin-right: 12px; position: relative; top: -3px; } .lft-border { border-left: 1px dotted #EBE4F6; padding-right: 12px; margin-left: 3px; box-sizing: border-box; padding-bottom: 12px; } /* .part .dec:last-child { border-bottom: 0; } */ .dec { margin: 10px 15px 0; padding-bottom: 10px; border-bottom: 1px dashed #EBE4F6; } .btm-border { border-left: 1px dashed #EBE4F6; } .text-bg { margin-top: 10px; background: #FFFBF1; padding: 14px; border-bottom: 0; position: relative; } .text-note-bg { margin-top: 10px; background: #FFFDF7; padding: 12px; border-bottom: 0; position: relative; } .text-note { width: 51px; height: 20px; line-height: 20px; background: #FFE2AA; text-align: center; border-radius: 0 0 8px 0; position: absolute; top: 0; left: 0; } .text-note-dec { margin-top: 15px;; } Induction of Type 2 Diabetes Mellitus (T2DM)[3][4][5] Background The disease is induced by partially destroying the animals' islet β cells, making the peripheral tissue insensitive to insulin, and by feeding them a high-calorie diet. Specific Mmodeling Methods Mice: C57BL/6 • female • 10 week-oldAdministration: • i.p. • high-fat diet+low-dose injection of 40 mg/kg STZ for 4 days.Rat: Sprague-Dawley or Wistar rats • male • 8-10 weeks-oldAdministration: i.p. • 8 weeks of high-fat diet+low-dose injection of 25 mg/kg STZ for 5 days. Note Tips: 1) The sensitivity of different species of animals to STZ varies greatly, and it is recommended to use male rats (female mice are more tolerant to STZ) [3];2) Fasting without water before administration can increase the sensitivity of pancreatic β cells to STZ. STZ injection in model animals generally requires rapid injection;3) Different strains of mice have different sensitivities to STZ. Studies have reported that the DBA/2 strain is the most sensitive, followed by C57BL6. Balb/cJ mice are resistant to multiple low-dose STZ-induced diabetes[4];4) After STZ treatment, animals die due to fatal hypoglycemia due to massive necrosis of pancreatic β-cells and sudden release of insulin, usually within 48 hours after injection. To prevent this, it is best to provide animals with 10% sucrose water regularly after STZ treatment. If animal mortality exceeds 20% when using a single high-dose STZ diabetic mouse protocol, treat animals with an intraperitoneal injection of 5% glucose solution within 6 hours of STZ injection[5];5) Preliminary experiments are required, and it is not recommended to directly use the administration methods and dosages in the literature. Modeling Indicators Blood glucose level : Blood glucose level exceeds 300 mg/dL(16.7 mmoL/L). Other indicators : generally accompanied by increased water intake, urine volume, and weight loss. Serum biochemical indexes such as total cholesterol, aspartate aminotransferase, triglyceride and low density lipoprotein also increased significantly with the occurrence of diabetes. Correlated Product(s): / Opposite Product(s): /

IC50 & Target：DNA alkylator[2] In Vitro The IC50 values of Streptozotocin for HL60, K562 and C1498 cells were 11.7, 904 and 1024 μg/ml, respectively[3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> Streptozotocin Related Antibodies

References：

[1]. Bennett RA, et al. Alkylation of DNA in rat tissues following administration of streptozotocin. Cancer Res. 1981 Jul;41(7):2786-90. [Content Brief]

[2]. Huang F, et al. Antidiabetic effect of a new peptide from Squalus mitsukurii liver (S-8300) in streptozocin-induced diabetic mice. J Pharm Pharmacol. 2005 Dec;57(12):1575-80. [Content Brief]

[3]. Diab RA, et al. Immunotoxicological effects of streptozotocin and alloxan: in vitro and in vivo studies. Immunol Lett. 2015 Feb;163(2):193-8. [Content Brief]

[4]. Furman BL. Streptozotocin-Induced Diabetic Models in Mice and Rats. Curr Protoc Pharmacol. 2015 Sep 1;70:5.47.1-5.47.20. [Content Brief]

[5]. Kim B, et al. Outbred Mice with Streptozotocin-Induced Diabetes Show Sex Differences in Glucose Metabolism. Int J Mol Sci. 2023 Mar 8;24(6):5210. [Content Brief]

[6]. Gurley SB, et al. Impact of genetic background on nephropathy in diabetic mice. Am J Physiol Renal Physiol. 2006 Jan;290(1):F214-22. [Content Brief]

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