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- LUTHOR - 3` mRNA-Seq Library Prep Kit
LUTHOR - 3` mRNA-Seq Library Prep Kit
LUTHOR combines a novel direct RNA amplification technology with an efficient one-step 3’ RNA-Seq library preparation method yielding unprecedented sensitivity and reproducibility for individual cells and purified RNA in the ultra-low pg range.
Unparalleled Sensitivity
LUTHOR 3’ mRNA-Seq offers unprecedented sensitivity for ultra-low input RNA and single cells compared to market leading commercial and academic protocols.
High Quality Performance for Ultra-low Input and Single Cells
LUTHOR 3’ mRNA-Seq reliably represents endogenous mRNA composition and efficiently excludes ribosomal rRNAs focusing sequencing reads on coding sequences.
Excellent Replicate and Cell-to-Cell Reproducibility
The combination of innovative THOR Amplification Technology and robust library generation delivers excellent reproducibility for replicates of ultra-low input RNA (Fig. 3A) and even for individual mES cells after freezing.
High Strand-Specificity
LUTHOR maintains exceptional strand-specificity of >99.9 % and allows to map reads to their corresponding strand on the genome, enabling the discovery and quantification of antisense transcripts and overlapping genes.
Cost Saving Multiplexing
LUTHOR 3’ mRNA-Seq library preps are intended to be used with Unique Dual Indices (UDIs). Lexogen offers 12 nt Unique Dual Indexing Sets with up to 384 pre-mixed UDIs in a convenient 96-well plate format. Lexogen’s new 12 nt UDIs feature superior error correction for maximal sequencing data output and are now also available as stand-alone Unique Dual Indexing Sets (Cat. No. 101 – 105, 156) for use with LUTHOR library preps.
Direct Counting for Gene Expression Quantification
Just one fragment per amplified antisense RNA copy is produced; therefore, no length normalization is required. This allows straightforward determination of gene expression values even at single cell level.
Mapping of Transcript End Sites
LUTHOR libraries contain inserts with an average size of ~150 base pairs. By using longer reads LUTHOR therefore allows to pinpoint the exact 3’ end of poly(A) RNA within single cells and to obtain accurate information about the 3’ UTR.
Simple Bioinformatics Analysis
Read mapping is simplified by skipping the junction detection. Reads are generated at the transcripts’ 3′ end where nearly no junctions are located. Data processing can hence be accelerated.
- The first comprehensive 3’ single-cell RNA-Seq
- Proprietary THOR Technology for direct RNA amplification
- Unprecedented sensitivity
- Empower challenging samples