Stemgent StemRNA - Model 00-0076 - 3rd Gen Reprogramming Kit
The StemRNA 3rd Gen Reprogramming Kit provides the fastest, most efficient method for generating clinically relevant iPS cells using a non-integrating, mRNA-based protocol. This technology supports generating iPSC lines derived from fibroblasts, blood, and urine using one multi-purpose kit.
The latest evolution in Stemgent RNA reprogramming, the StemRNA 3rd Gen Reprogramming Kit (formerly called the StemRNA-NM Reprogramming Kit), combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples.
- Flexible technology generates high-quality human iPS cell lines from multiple target cell types
Out-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs) and urine (urine-derived progenitor cells; UPCs) - High efficiency, non-integrating reprogramming
StemRNA-3rd Gen requires a few as four additional reagents. Double stranded microRNAs enhance reprogramming efficiency, providing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UPCs). - Time-saving protocol delivers faster results facilitating higher throughput
Colonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UPCs. No screening needed
Product Name: StemRNA 3rd Gen Reprogramming Kit
Catalog Number: 00-0076
Storage and Stability: Store all three kit components at or below −70 °C. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.
Quality Control: The individual mRNAs are tested for size and integrity. The StemRNA 3rd Gen Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts, human umbilical vein endothelial cells (HUVECs), EPCs, and UPCs. Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for Mycoplasma spp.
Recommended Usage: For use with associated StemRNA 3rd Gen Reprogramming Kit protocols for generation of iPS cells.
Kit Contents:
- OKSMNL NM-RNA (Part No. 05-0040), 30 µg, 1 vial
- EKB NM-RNA (Part No. 05-0041), 22 µg, 1 vial
- NM-microRNAs (Part No. 05-0042), 15 µg, 1 vial