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NanoTemper - Andromeda System
Poor expression levels, solubilization, and stability are well-known challenges in the production of recombinant membrane proteins. Selecting the best expression conditions typically focuses only on expression levels, while protein stability characterization is carried out separately, sometimes after protein purification. Andromeda increases the efficiency of CROs and Biopharma protein production teams by optimizing expression conditions and offering knowledge of expression levels and thermal stability from a single assay before protein purification.
Andromeda increases the efficiency of your protein production team
Screen more expression conditions faster in crude lysates
Routine workflows rely on more than one method to screen expression and extraction conditions — like SDS-PAGE and SEC or FSEC. Use Andromeda instead of these time-consuming and lower throughput methods. In a capillary format, analyze up to 48 crude lysates in just 11 minutes with little sample processing so you move on to scale-up and purification sooner.
Determine if your protein is highly expressed and properly folded in a single assay
To measure thermal stability, you either have to purify protein first or with crude lysates, you perform tedious, low throughput FSEC-TS. Andromeda easily identifies expression conditions that both yield sufficient and properly folded protein in crude lysates — because learning earlier if your protein is stable means not having to go back to screen more conditions.
Assess your protein’s binding activity by thermal shift assay before committing to purification
When producing a target protein for affinity screening studies, the expectation is you’ll deliver adequate amounts of structurally stable product. Andromeda quickly evaluates your protein’s thermal stability in the presence of ligands in crude lysates during small-scale expression.
Use Andromeda in your pharmaceutical protein production workflow
As a protein production team member, your job is to provide high-quality target proteins for biophysical characterization, affinity screening, and structural biology groups. Often, they put the quality of the proteins to the test — and when things don’t work as expected, your team is blamed.
Here’s what you can expect with Andromeda in your protein production workflow.
Save time by not having to repeat expression screening
If you know the stability of the protein early on, you purify the most stable target and save yourself the trouble of re-screening
Hand over high-quality proteins faster
Reduce the overall time it takes you to send purified proteins to the structural biology, biophysics, or screening teams by monitoring expression levels and thermal stability in one assay
Make informed decisions during low-scale expression
Evaluate not only expression levels but also stability early on, so you choose the right expression conditions before committing to purification