Aves - Model TUJ - Anti-Beta-Tubulin 3 Antibody

Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-GFAP, blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG. Image from Dr. Gerry Shaw (EnCor Biotechnology).

• Target Beta-Tubulin 3
• Target Description Human Beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.
• Format Affinity-Purified IgY
• Concentration 300 µg/mL
• Clonality Polyclonal
• Isotype IgY
• Applications ICC, IHC, WB
• Host Species Chicken
• Gene Name TUBB3 TUBB4
• Molecular Weight 50 kDa
• Antigen Synthetic peptides
• Species Reactivity Human, Mouse, Rat
• Antibody Registry ID AB_2313564
• Storage Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
• Physical State Liquid
• Production Notes Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Buffer Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
• Dilution Ranges WB: 1:10000-1:20000
• IHC: 1:2000-1:5000
• ICC: 1:2000-1:5000
• Quality Control Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
• Usage Statement These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
• Country of Origin United States
• Expiration 12 months from receipt of product
• Shipping Ice packs
• Synonyms Tubulin beta-3 chain (Tubulin beta-4 chain) (Tubulin beta-III)
• UniProt Details UniProt (Immunogen Species): Q13509