WOLF G2 - Benchtop Microfluidic Cell Sorter

• WOLFViewer software walks users though a simple workflow by offering step-by-step wizards to make the sorting process as straightforward as possible. As a result, the software uses an intuitive workflow allowing users to quickly complete setup.
• After setup, WOLFViewer makes analyzing and creating gates simple—while still providing full-featured flow cytometry tools.
• Key tools like automatic compensation and index sorting, are streamlined and included.
• When sorting is complete, shutdown and cleanup is literally less than a minute, allowing users to rapidly conclude their work.
• Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing through the WOLFViewer software.

The WOLF G2’s performance specifications allow users to complete various types of sorts for a variety of applications that may be occurring in their lab, for the ultimate in flexibility. The WOLF G2 can resolve lymphocytes, monocytes, and granulocytes, as well as microbial cells down to 1 μm, and up to 60 μm. Users can expect a sorting speed of up to 200 events per second maximally. Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing.

Scatter sensitivity: < 1.5 µm by FSC or BSC
Scatter Resolution: Resolves lymphocytes, monocytes, and granulocytes
Fluorescence sensitivity: < 200 MESF FITC (using 488 nm laser); < 250 MESF PE (using 561 nm laser)
Fluorescence Resolution: 8 peak separation with SPHERO™?; Rainbow Calibration Particles
Analysis Speed: > 5,000 events/second
Sorting: 1- and 2-way
Back-to-back Sorting Speed: 200 events per second
Absolute Counts: Yes
Volumetric Analysis: Yes
Warm-up Time: Less than 1 minute
Sorting Purity: Up to 99% purity
Sorting Output: 1.5 mL and 5.0 mL, 96- and 384-well plates

Physical Dimensions
The WOLF G2 is a simple, benchtop system that can easily be moved from lab-to-lab, and fits into biosafety cabinets. At 2 cubic feet and 54 pounds, researchers will not need to worry about the system taking up precious lab space.

WOLF Dimensions: 14.8H x 18.0W x 13.6D in (37.6H x 45.8W x 34.5D cm)
WOLF Weight: 54 lbs (24.5kg)
WOLF Electrical: AC Input: 100-240V, 50/60Hz, 0.5A
N1 Dimensions: 8.42W x 6.5H x 8.34D in (21.4 x 16.5 x 20.9)
N1 Weight: 5.5 lbs / 2.5 kg
N1 Electrical: DC Input: 24V 1A

Healthy Cells
At < 2 psi, the WOLF G2 are gentler than any conventional cell sorters, enabling healthier cells post-sort, especially for engineered lines, primary cells, and stem cells.

High Sensitivity and Resolution
All laser configurations afford < 250 MESF sensitivity, along with forward and back scatter, providing as low as 1 μm resolution.

Compact and at your bench
At 2 cubic feet, NanoCellect’s benchmark for access and performance allows every lab for the flexibility to do analysis and sorting into tubes or 96- and 384-well plates.

Simple and Intuitive
Intuitive software, fixed optics, no fluidics cart and less than one minute clean-up time.

Contaminant- and Biohazard-Free
Disposable, aerosol-free microfluidic cartridge allows for sterile sorting that protects the sample from the environment and scientist from the sample.

Expanding the WOLF’s Capabilities
With two lasers and up to nine fluorescent channels, the WOLF G2 aligns with a broad set of research applications and experiments. Three different laser configurations allows options specific to your needs.

Cartridge based fluidics

NanoCellect utilizes unique and proprietary microfluidic sorting cartridges for bulk sorting and single-cell dispensing. The cartridges employ a piezoelectric actuator and operates at less than 2 psi allows for gentle sorting that preserves viability and RNA integrity. Cartridges have a 100% disposable fluidic pathway, making it ideal for labs with many samples where cross-contamination or biohazard cleanup is an issue. The microfluidic cartridges are sterile and produce no aerosols, making it some of the safest sorting on the market. The cartridges also allow for maximum flexibility by allowing users to sort using their own buffers and media and only consume about 50 mL of sheath fluid in 1 day.

Fluidics
Sample Input: 1.5 and 5.0 mL tubes
Sheath Input: 50 mL Conical Tubes
Sheath Fluid: PBS or Buffer of Choice
Sheath Fluid Usage: 7.5 mL/Hour
Sample Flow Rate: 24 ul/Minute
Sheath Flow Rate: 160 uL/Minute
Dead Volume: 50 uL
Minimum Sample Volume: 150 uL
Tubing Diameter (Inner): 200 to 500 um
Flow Cell: 200 x 70 um
Sample Pressure: < 2psi

Product Specs Table Optics
The WOLF G2 uses laser-based flow cytometry technology for detecting and sorting a variety of cell types, including mammalian, plant, fungal, and bacterial cells. The WOLF G2 utilizes typical Forward- (FSC) and Back- (BSC) scatter detectors—as well as 3 different laser configurations to broaden its capabilities. In combination with the N1 single cell dispenser, scientists can complete both bulk and single-cell sorting. A simple cytometer, with powerful impact, allows researchers to sort their own cells on their own bench.

Excitation: 488 nm & 637 nm or 488 nm & 561 nm or 405 nm & 488 nm
Laser Profile: 25 x 27 µm
Scatter Detection: Forward (0 Degrees, +/-15); Back (180 Degrees, +/-15)
Emission Detection: See WOLF G2 Configuration Guide
Optical Alignment: Fixed alignment, no customer maintenance required